- Cambridge MA, US Peter Cavanagh - Stanford CA, US Miles Rogers - Cambridge MA, US Kirsty A. McFarland - Melrose MA, US Melissa M. Sprachman - Somerville MA, US Nicole E. Raustad - Charlestown MA, US
International Classification:
C12N 9/12
Abstract:
Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3′ end of a growing single-stranded DNA polynucleotide.