Ming Lei Zhang

US Patent:

20110223617, Sep 15, 2011

Filed:

Jul 9, 2009

Appl. No.:

13/003332

Inventors:

Ming Zhang - Brooklyn NY, US

International Classification:

G01N 33/566

US Classification:

435 79, 436501, 435 71

Abstract:

The present invention relates to the quantitative measurement of auto-reactive antibodies in a patient sample. In particular, the present invention is directed to, inter alia, a method for predicting the degree of cardiovascular injury in a patient following an ischemic event, said method comprising: immobilizing anti-NMHC II antibody on a solid support; adding a lysate of cardiac tissue to the solid support so that antigens in the lysate are captured by the immobilized antibody; adding a biological sample from the patient to said solid support, and incubating said sample for a time sufficient for IgM autoantibodies in the biological sample to bind to antigens in the cardiac tissue lysate; contacting said solid support with an anti-IgM antibody; removing unbound labeled antibodies; and determining the level of anti NMHC II autoantibodies in the biological sample by measuring the amount of labeled anti-IgM antibody bound to the solid support, wherein elevated levels of anti-NMHC II autoantibodies compared to normal individuals at time of patient admission indicates an increased risk of injury. Such methods are useful, inter alia, in the prognosis and monitoring of cardiovascular diseases.

US Patent:

20210063401, Mar 4, 2021

Filed:

Nov 20, 2018

Appl. No.:

16/765343

Inventors:

- Baltimore MD, US
Kenneth W. Kinzler - Baltimore MD, US
Qing Wang - Owings Mills MD, US
Nickolas Papadopoulos - Towson MD, US
Ming Zhang - Owings Mills MD, US

International Classification:

G01N 33/574

Abstract:

This document provides methods and materials for identifying biomarkers (e.g., peptide biomarkers) that can be used to identify a mammal as having a disease (e.g., cancer). This document also provides methods and materials for identifying and/or treating cancer. For example, this document provides methods and materials for using one or more peptide fragments derived from a peptidyl-prolyl cis-trans isomerase A (PPIA) polypeptide to identify a mammal as having cancer (e.g., ovarian cancer).

US Patent:

20180291369, Oct 11, 2018

Filed:

Feb 28, 2018

Appl. No.:

15/908190

Inventors:

- Towson MD, US
Ming ZHANG - Owings Mills MD, US

Assignee:

MyOmicsDx, Inc - Towson MD

International Classification:

C12N 15/10

Abstract:

A method and kit for constructing a barcoded single-stranded DNA library are disclosed. The method includes preparing single-stranded DNA molecules each having a dephosphorylated 5′ end, ligating a first adaptor to a 3′ end of each single-stranded DNA molecule, and synthesizing a complementary strand of each single-stranded DNA molecule ligated to the first strand of the first adaptor. The kit includes the first adaptor having a first strand, which includes, from a 5′ end to a 3′ end, a phosphate group, a barcode sequence, and a first primer recognition sequence. The kit also includes a DNA ligase for a ligation between the 5′ end of the first strand of the first adaptor to each single-stranded DNA molecule, and a first primer for the synthesis of the complementary strand. The method allows for analysis of rare mutations and from nucleic acid samples of low quality and quantity.

US Patent:

20180291436, Oct 11, 2018

Filed:

Mar 4, 2018

Appl. No.:

15/911161

Inventors:

- Towson MD, US
Ming ZHANG - Owings Mills MD, US

Assignee:

MyOmicsDx, Inc - Towson MD

International Classification:

C12Q 1/6834
B01J 19/00
B01L 3/00
B01L 7/00

Abstract:

A kit and a method for enriching target nucleic acid sequences from a biological sample are disclosed. The method includes preparing, and contacting with the biological sample, a first RNA probe set and a second RNA probe set respectively targeting both of the two antiparallel strands of a duplex segment in each target nucleic acid sequence. Each RNA probe in the first RNA probe set and the second RNA probe set can be generated by chemical synthesis or by in vitro or in vivo transcription, and can be biotin-labelled to thereby allow capturing of the target nucleic acid sequences by magnetic beads labelled with streptavidin, or can be engineered to a microfluidic channel to facilitate the capturing. The method can be applied to capture double-stranded nucleic acid sequences or single-stranded nucleic acid sequences having duplex segments, and the nucleic acid sequences can include DNAs, RNAs, or DNA-RNA hybrid molecules.

US Patent:

20180043512, Feb 15, 2018

Filed:

Oct 21, 2017

Appl. No.:

15/789986

Inventors:

Ming Liang Zhang - Brooklyn NY, US
Ming Zhu Zhang - Brooklyn NY, US

International Classification:

B25B 13/46
B25B 17/00

Abstract:

A ratchet wrench includes a first and a second drive members ′ rotatably mounted in a first and a second heads ′ extended from a first and a second ends of a handle . The first drive member includes a square inner periphery adapted to slidably retain a drive shaft including a ″ drive fitting and a ⅜″ drive fitting , and the second drive member ′ includes a ″ drive hexagonal bit holder and ″ drive fitting , for providing combined functions of ordinary ratchet wrenches for turning fasteners. A first and a second ratcheting mechanisms are mounted in the first and the second ends of the handle for turning the first and the second drive members ′, by the handle

US Patent:

20160332284, Nov 17, 2016

Filed:

May 2, 2016

Appl. No.:

15/144344

Inventors:

Ming Liang Zhang - Brooklyn NY, US
Ming Zhu Zhang - Brooklyn NY, US

International Classification:

B25B 13/46
B25B 13/48

Abstract:

A ratchet wrench includes a first and a second drive members rotatably mounted in a first and a second heads extended from a first and a second ends of a handle The first drive member includes a square inner periphery adapted to slidably retain a drive shaft including a ″ drive fitting and a 3/8″ drive fitting and the second drive member ′ includes a ″ drive hexagonal bit holder and ″ drive fitting for providing combined functions of ordinary ratchet wrenches for turning fasteners. A first and a second ratcheting mechanisms are mounted in the first and the second ends of the handle for turning the first and the second drive members by the handle