Nam N Vu

US Patent:

20130244257, Sep 19, 2013

Filed:

Mar 2, 2013

Appl. No.:

13/783283

Inventors:

THE REGENTS OF THE UNIVERSITY OF THE REGENTS CALIFORNIA - , US
Arion F. Hadjioannou (Chatziioannou) - Los Angeles CA, US
Cong Fang - Newbury Park CA, US
Yanju Wang - Los Angeles CA, US
Nam T. Vu - Redondo Beach CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

G01N 21/75

US Classification:

435 772, 4352872

Abstract:

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometric P-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analog F-FAC by deoxycytidine kinase (dCK) isolated from cell lysates.