Patrick Gilles

US Patent:

20110257031, Oct 20, 2011

Filed:

Feb 11, 2011

Appl. No.:

13/026046

Inventors:

John BODEAU - San Mateo CA, US
Heinz BREU - Palo Alto CA, US
Kathleen PERRY - San Francisco CA, US
Adam HARRIS - Carlsbad CA, US
Patrick GILLES - Carlsbad CA, US
Miho Gilles - Carsbad CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C40B 30/04
C07H 21/04
C40B 40/06

US Classification:

506 9, 506 16, 536 2431

Abstract:

Provided herein are systems, compositions and methods for tracking, sorting and/or identifying sample polynucleotides using nucleic acid barcodes. The barcodes provided herein are oligonucleotides that are designed to be uniquely identifiable. The nucleic acid barcodes have properties that permit them to be sequenced with high accuracy and/or reduced error rates. In some embodiments, the nucleic acid barcodes are designed to have certain nucleotide sequences that make up overlapping dibase color positions (also called color positions). The order of the overlapping dibase color positions can be determined using fluorophore-encoded dibase probes in a fluorophore color calling scheme to give high fidelity reads.