Ping Jiang - San Diego CA, US Mingxu Xu - La Jolla CA, US Meng Yang - San Diego CA, US
Assignee:
Anticancer, Inc. - San Diego CA
International Classification:
C12Q001/68
US Classification:
435 6, 435455, 435366
Abstract:
Living cells can be stably modified to emit different colors from the cytoplasm and nucleus, thus permitting analysis of the status of said cells and the effect of agents on said cells either by visual or instrumentally-aided observation. These observations may be made, if desired, in real time. In addition, rates of proliferation and drug sensitivities can be determined in vitro in real time by the use of cells modified to express a single fluorescent protein and observing fluorescence intensity as a function of time.
Ping Jiang - San Diego CA, US Mingxu Xu - La Jolla CA, US Yuying Tan - San Diego CA, US
Assignee:
AntiCancer, Inc. - San Diego CA
International Classification:
C12Q 1/68
US Classification:
435 6, 435 9151, 435378
Abstract:
Methods of obtaining faithful expression libraries from tissue samples comprise extraction of RNA from intact tissue cultured in three-dimensional sponge-gel based histocultures.
Immunocompromised Rodents As Dual Color Tumor Models
Meng Yang - San Diego CA, US Eligio Reynoso - San Diego CA, US Mingxu Xu - San Diego CA, US Ping Jiang - San Diego CA, US
Assignee:
Anticancer, Inc. - San Diego CA
International Classification:
A01K 67/027 A01K 67/02
US Classification:
800 18, 800 13, 800 22
Abstract:
Immunocompromised rodents that have been modified to express a fluorescent protein in substantially all tissues are described. These rodents are useful as models for gene expression, tumor progression and angiogenesis. Also provided are model systems where heterologous tissues fluorescing in a first color are transplanted into hosts that have been modified to fluoresce in substantially all tissues with a second color.
Ming Zhao - San Diego CA, US Xiao-Ming Li - San Diego CA, US Meng Yang - San Diego CA, US Mingxu Xu - La Jolla CA, US Ping Jiang - San Diego CA, US Lingna Li - San Diego CA, US
International Classification:
A61K048/00 A61K049/00
US Classification:
424/009600, 424/093210
Abstract:
A method to follow the progress of tumor treatment in subjects utilizes bacteria that have been modified to express a fluorescent protein. The method can also monitor expression of genes associated with the bacteria that produce therapeutic agents during the course of treatment, optionally against a background of fluorescence generated by the tumor itself. The method permits visualization of the progress of treatment in live subjects so that treatments can be modified according to their efficacy.
Ping Jiang - San Diego CA, US Meng Yang - San Diego CA, US Mingxu Xu - La Jolla CA, US
International Classification:
C12Q001/68 G01N033/574 C07K014/435
US Classification:
435/007230, 435/325000, 530/350000
Abstract:
Methods of recovering desired living cells contained in a tissue sample are based on separation based on fluorescence of a fluorescent protein produced by the desired cells.
Angiogensis Models Using Nestin-Expressing Stem Cells To Image Nascent Blood Vessels
Yasuyuki Amoh - San Diego CA, US Lingna Li - San Diego CA, US Meng Yang - San Diego CA, US Ping Jiang - San Diego CA, US
Assignee:
AntiCancer, Inc. - San Diego CA
International Classification:
C12Q001/00 A01K067/027
US Classification:
435004000, 435325000, 800019000
Abstract:
The disclosed invention relates to the observation that nestin expression is a marker for endothelial cell proliferation. Nestin expression is particularly useful as a marker for angiogenesis, particularly for tumor-related angiogenesis. Specifically, nestin serves as an excellent endothelium marker for brain tumors such as gliomas, hemangioblastomas, Schwannomas, medulloblastomas, and meningiomas. Accordingly, the disclosed invention relates to the use of this marker as a basis to model angiogenic activity.
Whole Body Imaging Using Portable Observation Systems