Prachand Shrestha

US Patent:

20210246486, Aug 12, 2021

Filed:

Feb 22, 2021

Appl. No.:

17/181115

Inventors:

- Visalia CA, US
Prachand Shrestha - Visalia CA, US

Assignee:

EdeniQ, Inc. - Visalia CA

International Classification:

C12Q 1/34
G01N 33/487
B01D 11/02

Abstract:

Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.

US Patent:

20190276865, Sep 12, 2019

Filed:

Mar 21, 2019

Appl. No.:

16/360408

Inventors:

- Visalia CA, US
Sandra Jacobson - El Cajon CA, US
Kris Ramos - Sanger CA, US
Prachand Shrestha - Visalia CA, US

International Classification:

C12P 19/14
C13K 1/02
C07H 3/04
C12P 19/12
C12P 7/10
C13K 13/00
C12P 19/02

Abstract:

The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

US Patent:

20160032339, Feb 4, 2016

Filed:

Mar 14, 2014

Appl. No.:

14/775588

Inventors:

- Visalia CA, US
Sandra Jacobson - EI Cajon CA, US
Kris Ramos - Sanger CA, US
Prachand Shrestha - Visalia CA, US

International Classification:

C12P 19/14
C12P 19/02

Abstract:

The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

US Patent:

20150176034, Jun 25, 2015

Filed:

Mar 3, 2015

Appl. No.:

14/637157

Inventors:

- Visalia CA, US
Donna Santos - Visalia CA, US
Padmavathy Desai - Fresno CA, US
Prachand Shrestha - Visalia CA, US
Richard Root Woods - Three Rivers CA, US
Steven Le - Palo Alto CA, US

International Classification:

C12P 7/14

Abstract:

The present disclosure provides methods and compositions for reducing the viscosity of biomass process streams in an ethanol production process. The method comprises adding cellulase enzymes to a biomass feedstock that is fermented to produce ethanol, generating whole stillage and thin stillage streams from the post-fermentation biomass, and adding an additional enzyme or enzyme cocktail that reduces the viscosity of the whole stillage stream, thin stillage stream, concentrated thin stillage stream, and/or the syrup stream generated by evaporating the thin stillage.

US Patent:

20140206055, Jul 24, 2014

Filed:

Jan 24, 2014

Appl. No.:

14/163464

Inventors:

- Visalia CA, US
Donna Santos - Visalia CA, US
Padmavathy Desai - Fresno CA, US
Prachand Shrestha - Visalia CA, US
Richard Root Woods - Three Rivers CA, US

Assignee:

Edeniq, Inc. - Visalia CA

International Classification:

C12P 7/10

US Classification:

435165

Abstract:

The present disclosure provides methods and compositions for reducing the viscosity of biomass process streams in an ethanol production process. The method comprises adding cellulase enzymes to a biomass feedstock that is fermented to produce ethanol, generating whole stillage and thin stillage streams from the post-fermentation biomass, and adding an additional enzyme or enzyme cocktail that reduces the viscosity of the whole stillage stream, thin stillage stream, concentrated thin stillage stream, and/or the syrup stream generated by evaporating the thin stillage.