Richard J Hanson

US Patent:

61438790, Nov 7, 2000

Filed:

Jul 28, 1998

Appl. No.:

9/123848

Inventors:

Lawrence Que - Roseville MN
Richard S. Hanson - Falcon Heights MN
Leah M. T. Schnaith - Redwing MN

Assignee:

Regents of the University of Minnesota - Minneapolis MN

International Classification:

C07H 2100
C07H 2102
C07H 2104

US Classification:

536 231

Abstract:

The present invention provides a unique series of nucleotide cleaving agents and a method for cleaving a nucleotide sequence, whether single-stranded or double-stranded DNA or RNA, using and a cationic metal complex having at least one polydentate ligand to cleave the nucleotide sequence phosphate backbone to yield a hydroxyl end and a phosphate end.

US Patent:

51963396, Mar 23, 1993

Filed:

Dec 20, 1991

Appl. No.:

7/814202

Inventors:

Richard S. Hanson - Wayzata MN
John D. Lipscomb - Wayzata MN

Assignee:

BioTrol, Inc. - Chaska MN

International Classification:

C02F 300

US Classification:

435262

Abstract:

A method is disclosed for degradation of a halogenated hydrocarbon compound such as trichloroethylene (TCE) which utilizes a soluble methane monooxygenase or a bacterium comprising the monooxygenase. Methylosinus trichosporium OB3b is a soluble methane monooxygenase-producing bacterium which when cultivated by continuous culturing comprising exposing the bacterium to a continuous-flow gas mixture of air and methane in a ratio of about 25:1-1:20, respectively. Methylosinus trichosporium OB3b is capable of degrading TCE at rates from about 500-10,000 micromoles per hour per gram cells. The present method is useful to degrade halogenated hydrocarbon compounds such as TCE at initial concentrations up to 10,000 micromoles/l.

US Patent:

62618253, Jul 17, 2001

Filed:

Mar 20, 1991

Appl. No.:

7/673264

Inventors:

Richard S. Hanson - Wayzata MN
Michael C. Flickinger - St. Paul MN
Frederick J. Schendel - Falcon Heights MN
Michael V. Guettler - Waconia MN

Assignee:

Regents of the University of Minnesota - Minneapolis MN

International Classification:

C12N 120
C12N 1501

US Classification:

4352525

Abstract:

A method of producing amino acids by culturing an amino acid auxotroph of a biologically pure strain of a type I methylotrophic bacterium of the genus Bacillus which exhibits sustained growth at 50. degree. C. using methanol as a carbon and energy source and requiring vitamin B. sub. 12 and biotin is provided.

US Patent:

61107131, Aug 29, 2000

Filed:

Jan 22, 1997

Appl. No.:

8/787206

Inventors:

Richard S. Hanson - Wayzata MN
Michael C. Flickinger - St. Paul MN
Patricia Olson - Maple Plain MN
Won Hur - Lauderdale MN
Craig Bremmon - Roseville MN

Assignee:

Regents of the University of Minnesota - Minneapolis MN

International Classification:

C12P 1314

US Classification:

435110

Abstract:

A method of producing glutamic acid by culturing an amino acid auxotroph of a biologically pure strain of Bacillus methanolicus which exhibits sustained growth at 50. degree. C. using methanol as a carbon and energy source and requiring vitamin B. sub. 12 and biotin is provided.

US Patent:

48247863, Apr 25, 1989

Filed:

Nov 13, 1987

Appl. No.:

7/107244

Inventors:

Richard S. Hanson - Deephaven MN
Larry N. Allen - Excelsior MN

Assignee:

The Regents of the University of Minnesota - Minneapolis MN

International Classification:

C12N 120
C12N 1500

US Classification:

4352523

Abstract:

A cloning vehicle comprising: a replication determinant effective for replicating the vehicle in a non-C. sub. 1 -utilizing host and in a C. sub. 1 -utilizing host; DNA effective to allow the vehicle to be mobilized from the non-C. sub. 1 -utilizing host to the C. sub. 1 -utilizing host; DNA providing resistance to two antibiotics to which the wild-type C. sub. 1 -utilizing host is susceptible, each of the antibiotic resistance markers having a recognition site for a restriction endonuclease; a cos site; and a means for preventing replication in the C. sub. 1 -utilizing host. The vehicle is used for complementation mapping as follows. DNA comprising a gene from the C. sub. 1 -utilizing organism is inserted at the restriction nuclease recognition site, inactivating the antibiotic resistance marker at that site. The vehicle can then be used to form a cosmid structure to infect the non-C. sub. 1 -utilizing (e. g. , E.

US Patent:

54418879, Aug 15, 1995

Filed:

Apr 29, 1994

Appl. No.:

8/235193

Inventors:

Richard S. Hanson - Wayzata MN
John D. Lipscomb - Wayzata MN
Brian G. Fox - Madison WI

Assignee:

Regents of the University of Minnesota - Minneapolis MN
BioTrol, Inc. - Eden Prairie MN

International Classification:

C12S 1300
B09B 300
C12N 130
C02F 300

US Classification:

4352625

Abstract:

A method is disclosed for degradation of a halogenated hydrocarbon compound such as trichloroethylene (TCE) which utilizes a soluble methane monooxygenase or a bacterium comprising the monooxygenase. Methylosinus trichosporium OB3b is a soluble methane monooxygenase-producing bacterium which when cultivated by continuous culturing comprising exposing the bacterium to a continuous-flow gas mixture of air and methane in a ratio of about 25:1-1:20, respectively. Methylosinus trichosporium OB3b is capable of degrading TCE at rates from about 500-10,000 micromoles per hour per gram cells. The present method is useful to degrade halogenated hydrocarbon compounds such as TCE at initial concentrations up to 10,000 micromoles/l.

US Patent:

53169280, May 31, 1994

Filed:

Jun 24, 1992

Appl. No.:

7/905170

Inventors:

Michael C. Flickinger - Saint Paul MN
Richard S. Hanson - Wayzata MN
Frederick J. Schendel - Oakdale MN
Charles R. Anderson - Apple Valley MN
Paul R. August - Bloomington MN

Assignee:

Regents of the University of Minnesota - Minneapolis MN

International Classification:

C12P 740
C12P 754

US Classification:

435136

Abstract:

The invention provides a method for the direct biological production of calcium magnesium acetate using a novel combination of an aerobic thermophilic bacterium of the genus Bacillus, capable of supporting the sustained production of substantial quantities of acetic acid at low pH, high temperatures, and high salt concentrations. Strains of bacterium of the genus Bacillus selected and isolated for use in the present invention produce acetic acid in the presence of a neutralizing source of calcium and magnesium ions, such as dolomitic lime, to produce substantial quantities of calcium magnesium acetate.

US Patent:

60837284, Jul 4, 2000

Filed:

Oct 17, 1997

Appl. No.:

8/953265

Inventors:

Frederick J. Schendel - Oakdale MN
Richard Dillingham - Minneapolis MN
Richard S. Hanson - Wayzata MN
Konosuke Sano - Kawasaki, JP
Kazuhiko Matsui - Kawasaki, JP

Assignee:

Regents of the University of Minnesota - Minneapolis MN

International Classification:

C12P 1314
C12N 120

US Classification:

435110

Abstract:

A method of producing glutamic acid by culturing a biologically pure wild type Bacillus methanolicus which exhibits sustained growth at 50. degree. C. using methanol as a carbon and energy source and requiring vitamin B. sub. 12 and biotin is provided.

Address:

10849 Falling Water Ln UNIT C, Saint Paul, MN 55129

VIN:

JTJBK1BA0A2427213

Make:

LEXUS

Model:

RX 350

Year:

2010