Robert Doyce Potter

US Patent:

8541219, Sep 24, 2013

Filed:

Apr 22, 2010

Appl. No.:

12/765253

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 9/00
C12N 9/12
C07K 1/00
C07K 14/00
C07K 17/00
C12Q 1/68
C12P 19/34

US Classification:

435183, 435194, 435 61, 435 911, 530350

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20040209276, Oct 21, 2004

Filed:

Sep 15, 2003

Appl. No.:

10/661819

Inventors:

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsville MD, US
Jun Lee - San Diego CA, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12N015/74

US Classification:

435/006000, 435/069100, 435/091200, 435/199000, 435/252300, 435/320100, 536/023200

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

US Patent:

20060030007, Feb 9, 2006

Filed:

Jun 1, 2004

Appl. No.:

10/857435

Inventors:

Devon Byrd - Fredericksburg VA, US
Dominic Esposito - Frederick MD, US
Robert Potter - San Marcos CA, US
Thomas Chappell - San Marcos CA, US

International Classification:

C07H 21/04
C12P 21/04
C12N 9/22
C07K 14/195

US Classification:

435069700, 435252300, 435320100, 435472000, 530350000, 536023500, 435199000

Abstract:

The invention relates generally to affinity peptides having binding activity for metal ion affinity chromatography media. The invention further relates to vectors which encode these affinity peptides and use of these affinity peptides for the purification of biological molecules such as proteins. The invention also relates to fusion proteins comprising affinity peptides of the invention.

US Patent:

20060094050, May 4, 2006

Filed:

Nov 3, 2005

Appl. No.:

11/265159

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12N 9/22

US Classification:

435006000, 435199000

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20060105348, May 18, 2006

Filed:

Nov 15, 2004

Appl. No.:

10/987453

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
Jonathan Nery - Escondido CA, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536025320

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

US Patent:

20060275792, Dec 7, 2006

Filed:

Feb 28, 2006

Appl. No.:

11/365383

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.

US Patent:

20060292578, Dec 28, 2006

Filed:

Jun 28, 2005

Appl. No.:

11/170762

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12P 19/34
C12N 9/22
C12N 15/74
C12N 1/21

US Classification:

435006000, 435069100, 435091200, 435199000, 435252300, 435471000, 536023200

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20070020622, Jan 25, 2007

Filed:

Sep 16, 2002

Appl. No.:

10/244081

Inventors:

Jun Lee - San Diego CA, US
Gary Gerard - Frederick MD, US
Harini Shandilya - Bethesda MD, US
Katherine Griffiths - Hornsby, AU
Moreland Gibbs - Marsfield, AU
Peter Bergquist - Auckland, NZ
Robert Potter - San Marcos CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12N 9/22

US Classification:

435006000, 435069100, 435199000, 435320100, 435325000, 536023200

Abstract:

The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may posses both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.