Ronald D Graham

Address:

212 N Kilworth St, Exira, IA 50076

VIN:

2CNALDEW7A6256589

Make:

CHEVROLET

Model:

EQUINOX

Year:

2010

US Patent:

6348596, Feb 19, 2002

Filed:

Jul 20, 1999

Appl. No.:

09/357740

Inventors:

Linda G. Lee - Palo Alto CA
Ronald J. Graham - Pleasanton CA
Khairuzzaman B. Mullah - Union City CA
Francis T. Haxo - San Francisco CA

Assignee:

PE Corporation (NY) - Foster City CA

International Classification:

C07D29300

US Classification:

546 75, 546 87, 546165, 546174, 5462701, 5462841, 435 6, 536 221, 536 253

Abstract:

The invention provides asymmetric cyanine dye compounds having the general formula: including substituted forms thereof, which are non-fluorescent quencher molecules. The invention further provides reporter-quencher dye pairs, wherein the asymmetric cyanine dyes are the quenchers, polynucleotides incorporating the asymmetric cyanine dyes, and nucleic acid hybridization detection methods utilizing the dye-labeled polynucleotides.

US Patent:

6372907, Apr 16, 2002

Filed:

Sep 14, 2000

Appl. No.:

09/661206

Inventors:

Linda G. Lee - Palo Alto CA
Ronald J. Graham - San Ramon CA
William E. Werner - San Carlos CA
Elana Swartzman - Alameda CA
Lily Lu - Foster City CA

Assignee:

Apptera Corporation - Foster City CA

International Classification:

C07D40500

US Classification:

546 41, 546289, 546304, 5462831, 549223, 549388, 525 541, 8563, 436800

Abstract:

The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.

US Patent:

6541618, Apr 1, 2003

Filed:

Jun 21, 2000

Appl. No.:

09/602544

Inventors:

Linda G. Lee - Palo Alto CA
Ronald J. Graham - Pleasanton CA
Khairuzzaman B. Mullah - Union City CA
Francis T. Haxo - San Francisco CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 2102

US Classification:

536 231, 536 253, 536 266, 548100, 548120, 548146, 548148, 548181, 548215, 548217, 548223

Abstract:

The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R and R is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.

US Patent:

6713622, Mar 30, 2004

Filed:

May 25, 2000

Appl. No.:

09/578920

Inventors:

Ronald J. Graham - Pleasanton CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 2100

US Classification:

536 2532, 536 221, 536253, 536 2531, 536 266, 435 6, 549227, 549356, 549381, 549385

Abstract:

A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R -R are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R and R is benzo, or, when taken together, R and R is benzo; R -R , R -R and R may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R and R may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y -Y are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y and R , Y and R Y and R , and/or Y and R is propano, ethano, or substituted forms thereof, and X -X taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

US Patent:

6750024, Jun 15, 2004

Filed:

Mar 31, 2003

Appl. No.:

10/404404

Inventors:

Linda G. Lee - Palo Alto CA
Ronald J. Graham - San Ramon CA
Khairuzzaman B. Mullah - Union City CA
Francis T. Haxo - San Francisco CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6, 536 231, 536 253, 536 266, 548100, 548120, 548146, 548148, 548181, 548215, 548217, 548223

Abstract:

The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R and R is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.

US Patent:

7074569, Jul 11, 2006

Filed:

May 28, 2004

Appl. No.:

10/856752

Inventors:

Sam Lee Woo - Redwood City CA, US
Ronald J. Graham - San Ramon CA, US
Jing Tian - Mountain View CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C07H 21/00
C07H 21/02

US Classification:

435 6, 536 231, 536 253, 536 266

Abstract:

The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

US Patent:

7166715, Jan 23, 2007

Filed:

May 5, 2004

Appl. No.:

10/840189

Inventors:

Linda G. Lee - Palo Alto CA, US
Ronald J. Graham - San Ramon CA, US
Khairuzzaman B. Mullah - Union City CA, US
Francis T. Haxo - San Francisco CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 21/02
C07H 19/04
C07D 293/00
C07D 421/00
C07D 293/10
C12Q 1/68

US Classification:

536 231, 536 253, 536 266, 548100, 548120, 548146, 548148, 548181, 548215, 548217, 548223, 435 6

Abstract:

The invention provides an asymmetric cyanine dye compound having the structure.

US Patent:

7256292, Aug 14, 2007

Filed:

Sep 16, 2005

Appl. No.:

11/229012

Inventors:

Ronald J. Graham - San Ramon CA, US
Ruiming Zou - Foster City CA, US
Krishna G. Upadhya - Union City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07D 219/06
C07D 221/18

US Classification:

546102, 546 61, 546 58

Abstract:

The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.