Stephanie D Hayes

US Patent:

20080057559, Mar 6, 2008

Filed:

Apr 18, 2007

Appl. No.:

11/785511

Inventors:

Laurette Nacamulli - Rockville MD, US
Jonathan Leland - Silver spring MD, US
Stephanie Hayes - Gaithersburg MD, US

International Classification:

C12N 9/00
G01N 31/00

US Classification:

435183000, 436002000

Abstract:

The rate of a biomolecular reaction, such as an enzymatic reaction or an affinity binding reaction, is measured using electrochemiluminescence (“ECL”). The reaction is conducted in an electrochemical cell with a mixture of reagents including a luminophore which will relate the concentration of a reactant, a reaction partner or the reaction product of a reaction partner to the ECL intensity. The reaction partner is a reagent which reacts with the reactant and which participates with the luminophore (or its reaction product participates with the luminophore) to cause the emission of ECL. The ECL intensity is modulated with a series of electrical pulses which are applied to the mixture of reagents at a preselected potential and for preselected intervals of time and duration. The ECL intensity is measured at the same intervals to provide a timed series of values (P). The same experiment is repeated except that the modulation is conducted after the reaction has gone to completion to obtain a timed series of values (C). The same experiment is repeated a third time in the absence of the reaction partner to obtain a times series of values (B). The results are normalized (N) using the following formula: to obtain a series of values N which can be used to plot the time course (concentration vs. time) of the reaction.

US Patent:

55277101, Jun 18, 1996

Filed:

Dec 2, 1994

Appl. No.:

8/347984

Inventors:

Laurette Nacamulli - Rockville MD
Jonathan K. Leland - Silver Spring MD
Stephanie A. Hayes - Gaithersburg MD

Assignee:

IGEN, Inc. - Gaithersburg MD

International Classification:

G01N 33557

US Classification:

436517

Abstract:

The rate of a biomolecular reaction, such as an enzymatic reaction or an affinity binding reaction, is measured using electrochemiluminescence ("ECL"). The reaction is conducted in an electrochemical cell with a mixture of reagents including a luminophore which will relate the concentration of a reactant, a reaction partner or the reaction product of a reaction partner to the ECL intensity. The reaction partner is a reagent which reacts with the reactant and which participates with the luminophore (or its reaction product participates with the luminophore) to cause the emission of ECL. The ECL intensity is modulated with a series of electrical pulses which are applied to the mixture of reagents at a preselected potential and for preselected intervals of time and duration. The ECL intensity is measured at the same intervals to provide a timed series of values (P). The same experiment is repeated except that the modulation is conducted after the reaction has gone to completion to obtain a timed series of values (C).

US Patent:

59453443, Aug 31, 1999

Filed:

Dec 8, 1997

Appl. No.:

8/986709

Inventors:

Stephanie A. Hayes - Gaithersburg MD
Jonathan K. Leland - Silver Spring MD
David B. Talley - Olney MD

Assignee:

Igen International, Inc. - Gaithersburg MD

International Classification:

G01N 2176

US Classification:

436172

Abstract:

The invention is a method for conducting an electrochemiluminescent assay that includes contacting a sample containing an analyte of interest with a first electrical waveform, followed by contacting the sample with a second electrical waveform that has been modulated in an amount derived from a pre-determined gain extension factor.