Steven P Short

US Patent:

20220410162, Dec 29, 2022

Filed:

Jun 28, 2022

Appl. No.:

17/851416

Inventors:

- Pleasanton CA, US
Anthony MAKAREWICZ - Livermore CA, US
Steven SHORT - Pleasanton CA, US

International Classification:

B01L 3/00
G01N 15/10
G01N 15/14

Abstract:

Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

US Patent:

20220379320, Dec 1, 2022

Filed:

Mar 15, 2022

Appl. No.:

17/654938

Inventors:

- Emeryville CA, US
Eric D. Hobbs - Livermore CA, US
Steven W. Short - Pleasanton CA, US
Mark P. White - San Francisco CA, US
Daniele Malleo - San Jose CA, US

International Classification:

B03C 5/00
B03C 5/02
B03C 11/00
B01L 3/00

Abstract:

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

US Patent:

20220228210, Jul 21, 2022

Filed:

Dec 29, 2021

Appl. No.:

17/565047

Inventors:

- Pleasanton CA, US
Denis PRISTINSKI - Dublin CA, US
Preyas SHAH - Milpitas CA, US
Steven William SHORT - Pleasanton CA, US
Dieter WILK - San Jose CA, US
Siyuan XING - Newark CA, US

International Classification:

C12Q 1/6874
C12N 15/10

Abstract:

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

US Patent:

20200115703, Apr 16, 2020

Filed:

Dec 16, 2019

Appl. No.:

16/715119

Inventors:

- Pleasanton CA, US
Anthony MAKAREWICZ - Livermore CA, US
Steven SHORT - Pleasanton CA, US

International Classification:

C12N 15/10
B01L 3/00
G01N 15/14
G01N 15/10
C12Q 1/6806
G01N 21/64

Abstract:

Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.

US Patent:

20180334670, Nov 22, 2018

Filed:

Jul 10, 2018

Appl. No.:

16/031880

Inventors:

- Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Steven Short - Pleasanton CA, US

International Classification:

C12N 15/10
G01N 15/14
B01L 3/00
G01N 21/64

Abstract:

Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.

US Patent:

20170021366, Jan 26, 2017

Filed:

Dec 18, 2014

Appl. No.:

15/105849

Inventors:

- Emeryville CA, US
Eric D. Hobbs - Livermore CA, US
Steven W. Short - Pleasanton CA, US
Mark P. White - San Francisco CA, US
Daniele Malleo - San Jose CA, US

Assignee:

Berkeley Lights, Inc. - Emeryville CA

International Classification:

B03C 5/00
B03C 11/00
B01L 3/00
B03C 5/02

Abstract:

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

US Patent:

20150166326, Jun 18, 2015

Filed:

Dec 18, 2013

Appl. No.:

14/133361

Inventors:

- Emeryville CA, US
Eric D. Hobbs - Livermore CA, US
Steven W. Short - Pleasanton CA, US
Mark P. White - San Francisco CA, US

Assignee:

Berkeley Lights, Inc. - Emeryville CA

International Classification:

B81B 1/00
B03C 5/02
B03C 5/00

Abstract:

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.