Thomas L Neumann

US Patent:

20120105600, May 3, 2012

Filed:

Jun 8, 2009

Appl. No.:

12/999515

Inventors:

Michael G. Meyer - Phoenix AZ, US
J. Richard Rahn - Sammamish WA, US
Anna V. Tourovskaia - Mountlake Terrace WA, US
Julia Oi Yan Yu - Santa Clara CA, US
Christy A. Lancaster - Seattle WA, US
Thomas Neumann - Seattle WA, US
Mark E. Fauver - Seattle WA, US

International Classification:

H04N 13/02

US Classification:

348 50, 348E13074

Abstract:

A method for 3D imaging of a biologic object () in an optical tomography system where a subcellular structure of a biological object () is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object () is moved relatively to a microscope objective () to present varying angles of view and the labeled biological object () is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object () and the microscope objective () within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object () from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ().