Thomas P Rohr

US Patent:

54459710, Aug 29, 1995

Filed:

Dec 1, 1994

Appl. No.:

8/348780

Inventors:

Thomas E. Rohr - Gurnee IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33546
G01N 33553

US Classification:

436526

Abstract:

The present invention provides devices for performing binding assays. Such devices comprise (i) a reaction vessel where unbound and immobilized magnetically-labeled reagent are produced in relation to the amount of said analyte in said test sample; (ii) a separation means for partitioning said immobilized magnetically-labeled reagent and said bound magnetically-labeled reagent; (iii) a magnetic field generator means for the application of a magnetic field to said magnetically-labeled reagent; and (iv) a measurement means to assess the effect of said magnetic field on said magnetically-labeled reagent as a measure of the presence or amount of said analyte in said test sample. The device provided by the instant invention can run, for example, direct indirect, competitive, inhibition and sandwich assay formats.

US Patent:

55676286, Oct 22, 1996

Filed:

Jun 30, 1994

Appl. No.:

8/268471

Inventors:

Peter J. Tarcha - Lake Villa IL
Thomas E. Rohr - Gurnee IL
James J. Markese - Downers Grove IL
Therese Cotton - Ames IA
Bernard N. Rospendowski - Sandyhills, GB

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33553

US Classification:

436525

Abstract:

A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.

US Patent:

52664988, Nov 30, 1993

Filed:

Nov 7, 1991

Appl. No.:

7/790106

Inventors:

Peter J. Tarcha - Lake Villa IL
Thomas E. Rohr - Gurnee IL
Therese Cotton - Ames IA

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33553

US Classification:

436525

Abstract:

An assay for an analyte in a test sample that uses a ligand binding reaction between the analyte and a specific binding member or a binding member pair that includes one binding member having a Raman active reporter. The analyte can be assayed by measurement of the Raman scattering signal of the Raman-active reporter. The Raman scattering signal can be either a noon-enhanced Raman scattering signal or a Raman scattering signal that is enhanced by a surface capable of enhancing that Raman scattering signal.

US Patent:

59982241, Dec 7, 1999

Filed:

May 16, 1997

Appl. No.:

8/857440

Inventors:

Thomas E. Rohr - Gurnee IL
Tuan A. Elstrom - Lake Bluff IL
Lawrence V. Howard - Libertyville IL
Eric B. Shain - Glencoe IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33553

US Classification:

436526

Abstract:

Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.

US Patent:

54459729, Aug 29, 1995

Filed:

Jul 22, 1993

Appl. No.:

8/095888

Inventors:

Peter J. Tarcha - Lake Villa IL
Thomas E. Rohr - Gurnee IL
Therese Cotton - Ames IA
Gary E. Winter - Hanover Park IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33532
G01N 33533

US Classification:

436544

Abstract:

A Raman label which, upon radiation, produces a detectable Raman scattering signal in a ligand-binding assay for an analyte in a test sample. A conjugate containing a Raman label attached to a specific binding member.

US Patent:

62943421, Sep 25, 2001

Filed:

Sep 29, 1999

Appl. No.:

9/409045

Inventors:

Thomas E. Rohr - Gurnee IL
Tuan A. Elstrom - Lake Bluff IL
Lawrence V. Howard - Libertyville IL
Eric B. Shain - Glencoe IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3353

US Classification:

435 71

Abstract:

Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.

US Patent:

53765567, Dec 27, 1994

Filed:

Sep 11, 1992

Appl. No.:

7/944138

Inventors:

Peter J. Tarcha - Lake Villa IL
Thomas E. Rohr - Gurnee IL
James J. Markese - Downers Grove IL
Therese Cotton - Ames IA
Bernard N. Rospendowski - Glasgow, GB

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33553

US Classification:

436525

Abstract:

A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.