Tony Tran

Address:

1718 Galahad Ln, Arlington, TX 76014

VIN:

4T4BF3EK4AR070826

Make:

TOYOTA

Model:

CAMRY

Year:

2010

US Patent:

20070212695, Sep 13, 2007

Filed:

Jan 12, 2006

Appl. No.:

11/331599

Inventors:

Vissarion Aivazachvili - Oakland CA, US
Konrad Faulstich - Fremont CA, US
Tony Tran - Mountain House CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C07H 21/04
C12P 19/34

US Classification:

435006000, 435091200, 536024300

Abstract:

The current teachings are directed to compositions, methods, and kits for selectively amplifying and for detecting target sequences. In some embodiments, a circularizable probe and/or a probe pair are disclosed for selectively amplifying target sequences. Methods for selectively amplifying target sequences are also disclosed, as are methods for detecting selectively amplified target sequences. Certain embodiments of the disclosed methods comprise a circularizable probe, a probe pair, comprising a first probe and a second probe, or both. In certain embodiments, a multiplicity of different circularizable probes, a multiplicity of different probe sets, or a multiplicity of different circularizable probes and a multiplicity of different probe sets are provided to selectively amplify or to detect a multiplicity of different target sequences, typically in a multiplex reaction. According to certain disclosed methods, surrogates of the target sequences are selectively amplified, including without limitation ligated probes, first amplification products, second amplification products, or combinations thereof. In some embodiments, selectively amplified target sequences or their surrogates are detected, directly or indirectly, indicating the presence of the corresponding target sequence. Kits to facilitate the performance of the disclosed methods are also provided.

US Patent:

20100184159, Jul 22, 2010

Filed:

Jan 22, 2010

Appl. No.:

12/692557

Inventors:

Vissarion AIVAZACHVILI - Oakland CA, US
Konard Faulstich - Salem-Neufrach, DE
Tony Tran - Mountain House CA, US

Assignee:

APPLIED BIOSYSTEMS, LLC - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 915

Abstract:

The current teachings are directed to compositions, methods, and kits for selectively amplifying and for detecting target sequences. In some embodiments, a circularizable probe and/or a probe pair are disclosed for selectively amplifying target sequences. Methods for selectively amplifying target sequences are also disclosed, as are methods for detecting selectively amplified target sequences. Certain embodiments of the disclosed methods comprise a circularizable probe, a probe pair, comprising a first probe and a second probe, or both. In certain embodiments, a multiplicity of different circularizable probes, a multiplicity of different probe sets, or a multiplicity of different circularizable probes and a multiplicity of different probe sets are provided to selectively amplify or to detect a multiplicity of different target sequences, typically in a multiplex reaction. According to certain disclosed methods, surrogates of the target sequences are selectively amplified, including without limitation ligated probes, first amplification products, second amplification products, or combinations thereof. In some embodiments, selectively amplified target sequences or their surrogates are detected, directly or indirectly, indicating the presence of the corresponding target sequence. Kits to facilitate the performance of the disclosed methods are also provided.