Angela E Higgins

US Patent:

20190250177, Aug 15, 2019

Filed:

Dec 4, 2018

Appl. No.:

16/208739

Inventors:

- Cleveland OH, US
Cory Bystrom - Beachwood OH, US
Angela Higgins - Cleveland OH, US

International Classification:

G01N 33/92
C07K 14/775
G01N 1/34

Abstract:

The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

US Patent:

20150331000, Nov 19, 2015

Filed:

May 15, 2015

Appl. No.:

14/713046

Inventors:

- Cleveland OH, US
Cory Bystrom - Beachwood OH, US
Angela Higgins - Cleveland OH, US

International Classification:

G01N 33/92
G01N 1/34

Abstract:

The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.