Gary M Pollack

US Patent:

20050032243, Feb 10, 2005

Filed:

Jul 25, 2002

Appl. No.:

10/484676

Inventors:

Gary Pollack - Chapel Hill NC, US

International Classification:

G01N033/543

US Classification:

436518000

Abstract:

A method for evaluating binding of a ligand to a target protein. The method includes the steps of: (a) providing a sample comprising a target protein and a ligand, wherein the target protein and ligand are suspected to be bound reversibly together in a complex; (b) preconditioning activated charcoal with the target protein; (c) contacting the sample with the preconditioned activated charcoal for a time sufficient to allow for adsorption of unbound ligand to the activated charcoal; (d) eluting the sample from the activated charcoal; and (e) determining an amount of ligand in the eluted sample to thereby evaluate binding of the ligand to the target protein. An apparatus useful in carrying out the method, and a method of making the same, are also disclosed.