Klint A Rose

US Patent:

7025323, Apr 11, 2006

Filed:

Sep 21, 2001

Appl. No.:

09/957894

Inventors:

Peter A. Krulevitch - Pleasanton CA, US
William J. Benett - Livermore CA, US
Klint A. Rose - Livermore CA, US
Julie Hamilton - Tracy CA, US
Mariam Maghribi - Davis CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

F16K 31/124

US Classification:

251 11, 251 63, 417490, 222263

Abstract:

Low power integrated pumping and valving arrays which provide a revolutionary approach for performing pumping and valving approach for performing pumping and valving operations in microfabricated fluidic systems for applications such as medical diagnostic microchips. Traditional methods rely on external, large pressure sources that defeat the advantages of miniaturization. Previously demonstrated microfabrication devices are power and voltage intensive, only function at sufficient pressure to be broadly applicable. This approach integrates a lower power, high-pressure source with a polymer, ceramic, or metal plug enclosed within a microchannel, analogous to a microsyringe. When the pressure source is activated, the polymer plug slides within the microchannel, pumping the fluid on the opposite side of the plug without allowing fluid to leak around the plug. The plugs also can serve as microvalves.

US Patent:

7186352, Mar 6, 2007

Filed:

May 25, 2004

Appl. No.:

10/853859

Inventors:

Jeffrey D. Morse - Martinez CA, US
Klint A Rose - Boston MA, US
Mariam Maghribi - Livermore CA, US
William Benett - Livermore CA, US
Peter Krulevitch - Pleasanton CA, US
Julie Hamilton - Tracy CA, US
Robert T. Graff - Modesto CA, US
Alan Jankowski - Livermore CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

H01M 8/00

US Classification:

216 67, 296234, 156 8912

Abstract:

Described herein is a process for fabricating microfluidic systems with embedded components in which micron-scale features are molded into the polymeric material polydimethylsiloxane (PDMS). Micromachining is used to create a mold master and the liquid precursors for PDMS are poured over the mold and allowed to cure. The PDMS is then removed form the mold and bonded to another material such as PDMS, glass, or silicon after a simple surface preparation step to form sealed microchannels.

US Patent:

7601286, Oct 13, 2009

Filed:

Mar 26, 2002

Appl. No.:

10/107933

Inventors:

William Benett - Livermore CA, US
Peter Krulevitch - Pleasanton CA, US
Mariam Maghribi - Livermore CA, US
Julie Hamilton - Tracy CA, US
Klint Rose - Boston MA, US
Amy W. Wang - Oakland CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

B29C 33/76

US Classification:

264221, 264317

Abstract:

A method of forming a polymer-based microfluidic system platform using network building blocks selected from a set of interconnectable network building blocks, such as wire, pins, blocks, and interconnects. The selected building blocks are interconnectably assembled and fixedly positioned in precise positions in a mold cavity of a mold frame to construct a three-dimensional model construction of a microfluidic flow path network preferably having meso-scale dimensions. A hardenable liquid, such as poly (dimethylsiloxane) is then introduced into the mold cavity and hardened to form a platform structure as well as to mold the microfluidic flow path network having channels, reservoirs and ports. Pre-fabricated elbows, T's and other joints are used to interconnect various building block elements together. After hardening the liquid the building blocks are removed from the platform structure to make available the channels, cavities and ports within the platform structure.

US Patent:

8524064, Sep 3, 2013

Filed:

Jul 26, 2010

Appl. No.:

12/843269

Inventors:

Dietrich A. Dehlinger - Dublin CA, US
Klint A. Rose - Alviso CA, US
Maxim Shusteff - Oakland CA, US
Christopher G. Bailey - Pleasanton CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01N 27/26
G01N 27/453
B03C 5/02
B01D 57/02

US Classification:

204643, 204547, 204400, 204600

Abstract:

A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.

US Patent:

20030203271, Oct 30, 2003

Filed:

Apr 24, 2002

Appl. No.:

10/131846

Inventors:

Jeffrey Morse - Martinez CA, US
Klint Rose - Boston MA, US
Mariam Maghribi - Livermore CA, US
William Benett - Livermore CA, US
Peter Krulevitch - Pleasanton CA, US
Julie Hamilton - Tracy CA, US
Robert Graff - Modesto CA, US
Alan Jankowski - Livermore CA, US

Assignee:

The Regents of the University of California

International Classification:

H01M008/02
H01M008/10
H01M004/86

US Classification:

429/038000, 429/030000, 429/026000, 429/042000, 029/623400

Abstract:

Described herein is a process for fabricating microfluidic systems with embedded components in which micron-scale features are molded into the polymeric material polydimethylsiloxane (PDMS). Micromachining is used to create a mold master and the liquid precursors for PDMS are poured over the mold and allowed to cure. The PDMS is then removed form the mold and bonded to another material such as PDMS, glass, or silicon after a simple surface preparation step to form sealed microchannels.

US Patent:

20110036718, Feb 17, 2011

Filed:

Aug 17, 2009

Appl. No.:

12/542215

Inventors:

Byoungsok Jung - San Jose CA, US
Kevin Ness - San Francisco CA, US
Klint A. Rose - Alviso CA, US

International Classification:

G01N 27/447

US Classification:

204549

Abstract:

According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

US Patent:

20110124098, May 26, 2011

Filed:

Nov 10, 2010

Appl. No.:

12/943677

Inventors:

Klint A. Rose - Alviso CA, US
Christopher G. Bailey - Pleasanton CA, US
Kevin Dean Ness - San Mateo CA, US

International Classification:

C12M 1/12

US Classification:

4353061

Abstract:

A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

US Patent:

20110250467, Oct 13, 2011

Filed:

Apr 12, 2011

Appl. No.:

13/085405

Inventors:

Klint Aaron Rose - Alviso CA, US
Joshua D. Kuntz - Livermore CA, US
Marcus Worsley - Hayward CA, US

Assignee:

LAWRENCE LIVERMORE NATIONAL SECURITY, LLC - Livermore CA

International Classification:

B32B 5/14
C22C 21/00
C04B 35/10
B32B 7/02
B32B 18/00
C25D 13/00
B32B 3/10

US Classification:

428610, 204477, 204478, 204484, 420528, 4281951, 428218, 428325, 501127

Abstract:

A ceramic, metal, or cermet according to one embodiment includes a first layer having a gradient in composition, microstructure and/or density in an x-y plane oriented parallel to a plane of deposition of the first layer. A ceramic according to another embodiment includes a plurality of layers comprising particles of a non-cubic material, wherein each layer is characterized by the particles of the non-cubic material being aligned in a common direction. Additional products and methods are also disclosed.