Peter L Lengyel

US Patent:

20130302899, Nov 14, 2013

Filed:

Jul 12, 2013

Appl. No.:

13/940609

Inventors:

Andrew J. Murphy - Croton-on-Hudson NY, US
Peter Matthew Lengyel - Brooklyn NY, US
Peter H.A. Yang - Tarrytown NY, US

International Classification:

C12N 15/90

US Classification:

435462, 4353201

Abstract:

Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.

US Patent:

20110104799, May 5, 2011

Filed:

Oct 29, 2010

Appl. No.:

12/915447

Inventors:

Aris N. Economides - Tarrytown NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US
Peter Matthew Lengyel - Brooklyn NY, US
Peter H.A. Yang - Tarrytown NY, US

Assignee:

Regeneron Pharmaceuticals, Inc. - Tarrytown NY

International Classification:

C12N 5/10
C12N 15/85

US Classification:

435325, 4353201

Abstract:

Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.

US Patent:

20220220510, Jul 14, 2022

Filed:

Apr 1, 2022

Appl. No.:

17/712003

Inventors:

- Tarrytown NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US
Peter Matthew Lengyel - Brooklyn NY, US
Peter H.A. Yang - Tarrytown NY, US

Assignee:

REGENERON PHARMACEUTICALS, INC. - Tarrytown

International Classification:

C12N 15/90
C12N 9/00
C12N 15/63

Abstract:

Methods for modifying a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising conditional by inversion elements. Methods are provided which afford the ability in a single targeting step to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a conditional by inversion element. Introduced alleles comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a conditional by inversion element, wherein upon action of a recombinase a conditional allele with a conditional by inversion element is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a conditional by inversion element in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.

US Patent:

20190390230, Dec 26, 2019

Filed:

Jul 1, 2019

Appl. No.:

16/459236

Inventors:

- TARRYTOWN NY, US
Andrew J. Murphy - Croton-on-Hudson NY, US
Peter Matthew Lengyel - Brooklyn NY, US
Peter H.A. Yang - Tarrytown NY, US

Assignee:

REGENERON PHARMACEUTICALS, INC. - TARRYTOWN NY

International Classification:

C12N 15/90
C12N 15/63
C12N 9/00

Abstract:

Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation.