Stanley J Thomas

US Patent:

51069660, Apr 21, 1992

Filed:

Jul 10, 1989

Appl. No.:

7/381588

Inventors:

Stanley Thomas - Overland Park KS
Lowell Tilzer - Overland Park KS
Ruben Moreno - Overland Park KS

Assignee:

University of Kansas - Kansas City KS

International Classification:

C07H 2100
C07H 2104

US Classification:

536 27

Abstract:

The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a polyester gel polymer to the mixture of sample and separating reagent followed by agitation via, e. g. , centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.

US Patent:

51752719, Dec 29, 1992

Filed:

Jan 27, 1992

Appl. No.:

7/825695

Inventors:

Stanley Thomas - Overland Park KS
Lowell Tilzer - Overland Park KS
Ruben Moreno - Overland Park KS

Assignee:

University of Kansas - Kansas City KS

International Classification:

C07H 2100
C07H 2104

US Classification:

536 27

Abstract:

The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a silical gel polymer to the mixture of sample and separating reagent followed by agitation via, e. g. , centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.