Zhiqiang E Han

US Patent:

20090163431, Jun 25, 2009

Filed:

Feb 14, 2008

Appl. No.:

12/031139

Inventors:

Irene Kazhdan - San Antonio TX, US
Zhiqiang Han - Fenton MO, US
Zheng Jim Wang - San Antonio TX, US
Ting-Tung Chang - San Antonio TX, US
Robert A. Marciniak - San Antonio TX, US

Assignee:

Ontherex LLC - San Antonio TX

International Classification:

A61K 31/7088
C12N 5/06
C12N 15/00

US Classification:

514 44, 435375, 4353201

Abstract:

Methods and compositions for inhibiting PDX-1 are provided according to the present invention. An anti-PDX-1 agent included in inventive methods and compositions includes an antibody, an aptamer, an antisense oligonucleotide, a ribozyme and/or an inhibitory compound. Methods of inhibiting PDX-1 expression in a tumor cell are provided by the present invention which include contacting a tumor cell with an effective amount of an anti-PDX- agent highly or completely complementary to a specified region of an RNA molecule encoding PDX-1. Such an agent specifically hybridizes with the RNA molecule encoding PDX-1 and inhibits the expression of a PDX-1 gene in the tumor cell. Compositions including anti-PDX-1 siRNA and/or shRNA are described. Recombinant expression constructs encoding anti-PDX-1 siRNA or shRNA according to the present invention are described.