Addison D Ault

US Patent:

20080070794, Mar 20, 2008

Filed:

May 21, 2007

Appl. No.:

11/804905

Inventors:

James Broach - Skillman NJ, US
Addison Ault - Princeton NJ, US
Ho Fung - Princeton NJ, US
Christodoulos Floudas - Princeton NJ, US

International Classification:

C40B 30/04
C40B 70/00

US Classification:

506009000, 506041000

Abstract:

In one embodiment the invention provides a mutant UDP-glucose receptor (P2Y14) functionally expressed in the yeast . The mutant receptors have ligand-binding properties that are useful as practical biosensors. Mutagenesis of the entire UDP-glucose receptor gene yielded receptors with increased activity but similar ligand specificities, while random mutagenesis of residues in the immediate vicinity of the ligand-binding pocket yielded mutants with altered ligand specificity. The receptor mutants can be used to detect chemical ligands in complex mixtures and to discriminate among chemically or stereochemically related compounds. Also provided are methods for combinatorial applications wherein engineered receptors can be applied, for example, in a pairwise manner to differentiate among several chemical analytes that would be indistinguishable with a single receptor.

US Patent:

20110177964, Jul 21, 2011

Filed:

Dec 3, 2010

Appl. No.:

12/960157

Inventors:

James R. Broach - Skillman NJ, US
Addison D. Ault - Pennington NJ, US
Alexandre V. Morozov - Princeton NJ, US
Julia Tsitron - Brooklyn NY, US

Assignee:

THE TRUSTEES OF PRINCETON UNIVERSITY - Princeton NJ
Rutgers, The State University of New Jersey - New Brunswick NJ

International Classification:

C40B 30/04
C40B 60/12
C40B 50/00
C40B 40/10

US Classification:

506 9, 506 39, 506 23, 506 18

Abstract:

A chemosensor array, a method of detecting ligands in a chemical mixture and a chemosensor system are provided. Chemosensor array designs are also provided.

US Patent:

20070154947, Jul 5, 2007

Filed:

Aug 31, 2006

Appl. No.:

11/513594

Inventors:

James Broach - Skillman NJ, US
Addison Ault - Princeton NJ, US

International Classification:

G01N 33/53
G01N 33/569
C07K 16/28

US Classification:

435007100, 435007310, 530388220

Abstract:

In one embodiment the invention provides a mutant UDP-glucose receptor (P2Y14) functionally expressed in the yeast The mutant receptors have ligand-binding properties that are useful as practical biosensors. Mutagenesis of the entire UDP-glucose receptor gene yielded receptors with increased activity but similar ligand specificities, while random mutagenesis of residues in the immediate vicinity of the ligand-binding pocket yielded mutants with altered ligand specificity. The receptor mutants can be used to detect chemical ligands in complex mixtures and to discriminate among chemically or stereochemically related compounds. Also provided are methods for combinatorial applications wherein engineered receptors can be applied, for example, in a pairwise manner to differentiate among several chemical analytes that would be indistinguishable with a single receptor.