IDERA PHARMACEUTICALS, INC Biological Research & Development · Mfg Biological Products and Pharmaceutical Preparations · Mfg of Biological Products and Research and Development · Druggists' Goods Merchant Whols
167 Sidney St, Cambridge, MA 02139 (617)6795500, (617)2250078, (617)4989052, (617)6795592
Us Patents
Electrophoretic Detection And Separation Of Mutant Dna Using Replaceable Polymer Matrices
Barry L. Karger - Newton MA William G. Thilly - Winchester MA Frantisek Foret - Malden MA Aharon S. Cohen - Newton MA Roger W. Giese - Quincy MA
Assignee:
Massachusetts Institute of Technology - Cambridge MA Northeastern University - Boston MA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.
Substrate Useful For Separating Modified Oligonucleotides
Aharon S. Cohen - Brookline MA Maria Vilenchik - Natick MA
Assignee:
Hybridon, Inc. - Worcester MA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
Disclosed is a substrate useful for separating unmodified and modified mononucleotides and oligonucleotides. The substrate includes at least 12% polymer in at least 50% (volume:volume) organic solvent, the organic solvent being a denaturing agent. This substrate is easily removable from the capillary using low pressure. Also provided is a method of separating unmodified and modified mononucleotides and oligonucleotides utilizing this substrate.
Aharon S. Cohen - Brookline MA Alexei Belenky - Newton MA Christopher M. Ott - Oakham MA
Assignee:
Hybridon, Inc. - Worcester MA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
Disclosed is a method for determining the nucleotide sequence of a target oligonucleotide. In this method a single-stranded ligation product is prepared which contains a target oligonucleotide-to-be sequenced and an auxiliary oligonucleotide. A primer complementary to a portion of the auxiliary oligonucleotide and having a label covalently attached thereto is annealed to the auxiliary oligonucleotide portion of the ligation product. The primer is extended with chain-extending nucleoside triphosphates and chain-terminating nucleoside triphosphates in the presence of a polymerase to yield a plurality of primer extension products. These extension products are then separated on the basis of their base length; and the nucleoside sequence of the target oligonucleotide is determined from the mobilities of the primer extension products obtained during their separation.
High Performance Microcapillary Gel Electrophoresis
Barry L. Karger - Newton MA Aharon S. Cohen - Brookline MA
Assignee:
Northeastern University - Boston MA
International Classification:
G01N 2726 B01D 5702
US Classification:
2041828
Abstract:
A microcapillary column for high performance electrophoresis. A preferred column includes a microcapillary, a thin layer of coating material covalently bonded to the inner surface of the microcapillary wall, and a gel comprising polyacrylamide polymerized in the tube, filling it. The gel-containing microcapillary is prepared by covalently bonding a layer of a suitable coating material to the inner surface of the microcapillary wall, and then causing a mixture of monomer with or without crosslinking agent, initiator, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates and in some instances over 1,000,000 theoretical plates within separation times of less than thirty minutes, permits trace level determinations of molecular weights, and permits electrophoretic operation at fields of 300 V/cm or higher, resulting in extremely high resolution separations.
Method For Detecting Charged Oligonucleotides In Biological Fluids
Aharon S. Cohen - Brookline MA Andre Bourque - Marlboro MA
Assignee:
Hybridon, Inc. - Worcester MA
International Classification:
C12Q 168 G01N 3353 C07H 2100
US Classification:
435 6
Abstract:
Disclosed is a method for detecting and quantitating oligonucleotides with charged internucleotide linkages in biological fluids. In this method, a biological fluid sample is contacted with an anion exchange resin at from 40. degree. C. to 65. degree. C. for a time sufficient to enable oligonucleotides in the sample to adsorb to the resin. The absorbed oligonucleotides are then desorbed with a buffer having a salt concentration of about 1 M to 2. 5 M and a pH in the range of about 6. 5 to 7. 5, the desorption being performed at about 40. degree. -65. degree. C. The oligonucleotides so released are then detected and quantitated.
Selective High Performance Electrokinetic Separations Employing The Surface Of Moving Charged Colloidal Particles
Barry L. Karger - Newton MA Aharon S. Cohen - Brookline MA David N. Heiger - Medford MA
Assignee:
Northeastern University - Boston MA
International Classification:
C25B 100 C25B 700 B01D 6142
US Classification:
2041828
Abstract:
Apparatus and method for conducting electrophoresis in a capillary tube under the influence of a pulsed electric field. Apparatus for separating and detecting molecular species of a sample in a conductive medium includes a capillary tube for containing conductive medium, means for introducing a sample into a conductive medium-filled capillary tube, means for applying a pulsed electric field across the conductive medium-filled capillary tube between its ends, and means for detecting constituent molecular species of a sample in a conductive medium-filled capillary tube as they traverse the tube. A method for separating and detecting molecular species of a sample in a conductive medium includes the steps of introducing the sample into a capillary tube filled with conductive medium, applying a pulsed electric field of up to 500 volts/centimeter between the ends of the capillary, and detecting the constituent molecular species of the sample as they traverse the capillary.
High Performance Microcapillary Gel Electrophoresis
Barry L. Karger - Chestnut Hill MA Aharon Cohen - Brookline MA
Assignee:
Northeastern University - Boston MA
International Classification:
G01N 2728 G01N 2726
US Classification:
2041828
Abstract:
A microcapillary column for high performance electrophoresis includes a fused silica microcapillary, a gel of crosslinked polyacrylamide polymerized in the tube, and a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The gel-containing microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates within separation times of less than thirty minutes, and permits trace level determinations of molecular weights.