Ailen M Sanchez

US Patent:

7326557, Feb 5, 2008

Filed:

Nov 12, 2004

Appl. No.:

10/987265

Inventors:

Ka-Yiu San - Houston TX, US
George N. Bennett - Houston TX, US
Ailen Sanchez - Houston TX, US

Assignee:

Rice University - Houston TX

International Classification:

C12N 1/12
C12N 15/63
C12Q 1/68
G01N 33/554
C07H 21/02

US Classification:

4352523, 435 6, 435 732, 4353201

Abstract:

A method of increasing cellular NADPH levels by expressing one or more genes that encode a enzyme that causes the production of NADPH. The system can be combined with other enzymes that require NADPH, thus improving the overall production of the desired protein.

US Patent:

7569380, Aug 4, 2009

Filed:

Dec 22, 2005

Appl. No.:

11/315453

Inventors:

Ka-Yiu San - Houston TX, US
Ailen Sanchez - Houston TX, US
George N. Bennett - Houston TX, US
Cheryl Renee Dittrich - Houston TX, US

Assignee:

Rice University - Houston TX

International Classification:

C12N 1/20
C12N 5/00
C12N 5/02

US Classification:

4352523, 435325, 435383

Abstract:

In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

US Patent:

7790416, Sep 7, 2010

Filed:

Apr 5, 2007

Appl. No.:

11/697295

Inventors:

Ka-Yiu San - Houston TX, US
George N. Bennett - Houston TX, US
Ailen Sanchez - South San Francisco CA, US

Assignee:

Rice University - Houston TX

International Classification:

C12P 1/00
C12P 21/04
C12N 1/20
C12N 5/00

US Classification:

435 712, 435 41, 4352528, 435325

Abstract:

The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.

US Patent:

20050042736, Feb 24, 2005

Filed:

Aug 20, 2004

Appl. No.:

10/923635

Inventors:

Ka-Yiu San - Houston TX, US
George Bennett - Houston TX, US
Ailen Sanchez - Houston TX, US

International Classification:

C12P019/30
C12N015/85
C12P007/40

US Classification:

435089000, 435455000

Abstract:

The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.

US Patent:

20060046288, Mar 2, 2006

Filed:

Aug 29, 2005

Appl. No.:

11/214309

Inventors:

San Ka-Yiu - Houston TX, US
George Bennett - Houston TX, US
Ailen Sanchez - Houston TX, US

International Classification:

C12P 7/46
C12N 1/21

US Classification:

435145000, 435252330

Abstract:

The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.

US Patent:

20060073577, Apr 6, 2006

Filed:

Sep 16, 2005

Appl. No.:

11/228830

Inventors:

San Ka-Yiu - Houston TX, US
George Bennett - Houston TX, US
Lin Henry - Houston TX, US
Ailen Sanchez - Houston TX, US

International Classification:

C12P 13/04
C12P 7/40
C12N 1/21

US Classification:

435106000, 435136000, 435252300

Abstract:

The invention relates to a hybrid succinate production system that has a high capacity to produce succinate under aerobic and anaerobic conditions. The metabolic engineering of a hybrid bacterial succinate production system that can function under both aerobic and anaerobic conditions makes the production process more efficient, and the process control and optimization less difficult.

US Patent:

20080125580, May 29, 2008

Filed:

Jul 13, 2007

Appl. No.:

11/777997

Inventors:

Shelly Pizarro - San Carlos CA, US
Ailen Sanchez - Foster City CA, US
Charles H. Schmelzer - Burlingame CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C07K 1/00
C07K 1/14
C07K 1/36
C07K 14/475

US Classification:

530399, 530413, 530422, 530423

Abstract:

Processes are provided for recovering and purifying refolded recombinant proteins produced in heterologous host cells, which includes the step of refolding the protein in a high pH buffer.

US Patent:

20110294990, Dec 1, 2011

Filed:

Aug 2, 2011

Appl. No.:

13/196680

Inventors:

SHELLY PIZARRO - SAN CARLOS CA, US
AILEN SANCHEZ - FOSTER CITY CA, US
CHARLES H. SCHMELZER - BURLINGAME CA, US

Assignee:

GENENTECH, INC. - SOUTH SAN FRANCISCO CA

International Classification:

C07K 1/36
C07K 14/475
C07K 1/14

US Classification:

530399, 530412, 530416

Abstract:

Processes are provided for recovering and purifying refolded recombinant proteins produced in heterologous host cells, which includes the step of refolding the protein in a high pH buffer.