Alan M Beem

US Patent:

20160237425, Aug 18, 2016

Filed:

May 23, 2015

Appl. No.:

14/545581

Inventors:

Alan M.H. Beem - Seattle WA, US

International Classification:

C12N 15/113
C12N 15/10

Abstract:

Provided are methods for the design, preparation, and use of non-native pri-microRNAs (pri-miRNAs), pri-microRNA (pri-miRNA) scaffolds, and libraries of non-native pri-miRNAs employing pri-miRNA scaffolds. Also provided are methods for identifying non-native pri-miRNAs, combinations of two or more non-native pri-miRNAs, and miRNAs derived from the processing of such non-native pri-miRNAs, which miRNAs exhibit one or more desired functional activities. Further provided are non-native pri-miRNAs, non-native pri-miRNA libraries, vectors comprising and for the expression of one or more non-native pre-miRNAs or for the expression of one or more miRNAs derived from the processing of one or more pre-miRNAs, and cells comprising one or more non-native pri-miRNAs or one or more miRNAs derived from the processing of such non-native pri-miRNAs, each of which pri-miRNAs, pri-miRNA libraries, vectors, and cells can be prepared by the methods disclosed herein. Still further provided are (a) methods for regulating, promoting, normalizing, restoring, inhibiting, or modulating a desired cellular phenotype including, for example, differentiation, de-differentiation, proliferation, growth, cell death, contact inhibition by expressing one or more pri-miRNAs or one or more miRNAs identified through the screening of a pri-miRNA library according to the methodology disclosed herein and (b) methods for the treatment of a disease or condition that associated with the expression of one or more gene or the production of one or more protein, wherein one or more aspect of the disease or condition is reduced in severity following the expression of one or more pri-miRNAs or miRNAs identified through the screening of a pri-miRNA library according to the methodology disclosed herein.

US Patent:

20150337332, Nov 26, 2015

Filed:

Dec 18, 2013

Appl. No.:

14/652756

Inventors:

- Seattle WA, US
Pratyusha Banik - Bothell WA, US
Alan Beem - Seattle WA, US
Sandra Shannon - Sammamish WA, US
Henrik Sperber - Seattle WA, US

International Classification:

C12N 15/85

Abstract:

The present disclosure provides methods and compositions to selectively modulate RNA processing. The methods and compositions selectively enhance or repress RNA processing by up- or down-regulating Drosha expression and/or by providing RNA sequences with mis-matches introduced or removed 5 and/or 9-12 nucleotide positions from the Drosha cutting site. Therapeutic uses of the methods and compositions are also described.