Skills:
Technical Skills: Cell biology Mammosphere culture/ Spheroid suspension culture from primary cells Primary cell culture Cell line maintenance Cell line clone isolation and selection with antibiotics Transfection Lentiviral production and infection Treatment with small molecule inhibitor dugs Wounding and Boyden chamber assays to assess motility and invasion Induced differentiation Molecular Biology Western Blots qPCR and PCR Molecular cloning Yeast Two-hybrid screening Microscopy Immunofluorescence microscopy from multiple sample types Live cell microscopy Semi-automated image processing and statistical analysis Routine Microscope maintenance Metamorph experience Biochemistry Protein purification from e.coli Antibody purification using immobilized antigen Protein and antibody concentration Immunoprecipitation Quantitative data analysis Use of statistics including t-tests, ANOVA, CHI Square and confidence intervals Minitab and SPSS experience Animal handling and mouse colony maintenance Mouse breeding, weaning, and crossing mouse model lines Tumor palpation and observation including tumor weight over time Mouse tattooing Genotyping Other Specialized training includes Laboratory and Chemical Safety, Radiation Safety including gamma ray sources, Bioethics training, Grant writing