Alexander H Yang

US Patent:

20200392191, Dec 17, 2020

Filed:

Jul 10, 2020

Appl. No.:

16/925848

Inventors:

- Pleasanton CA, US
Tim CRAIG - Mountain View CA, US
Alexander YANG - Mountain View CA, US
Liv JENSEN - Mountain View CA, US
Charlotte YANG - Mountain View CA, US
Corissa HARRIS - Mountain View CA, US
Matthew DIPIETRO - Gilroy CA, US
Dhruti DALAL - Sunnyvale CA, US

Assignee:

Roche Sequencing Solutions, Inc. - Pleasanton CA

International Classification:

C07K 14/245
C12Q 1/6874
C12Q 1/68
G01N 33/68
C12N 9/12

Abstract:

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

US Patent:

20200325534, Oct 15, 2020

Filed:

Jun 30, 2020

Appl. No.:

16/917435

Inventors:

- Pleasanton CA, US
Cynthia Ann CECH - Newcastle WA, US
Michael DORWART - Mountain View CA, US
Liv Elisabeth JENSEN - Palo Alto CA, US
Marshall Winston PORTER - Santa Clara CA, US
Alexander Hyun-min YANG - Campbell CA, US

Assignee:

Roche Sequencing Solutions, Inc. - Pleasanton CA

International Classification:

C12Q 1/6869
C07K 14/31

Abstract:

Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

US Patent:

20200216887, Jul 9, 2020

Filed:

Jan 20, 2017

Appl. No.:

16/071406

Inventors:

- Santa Clara CA, US
- Mannheim, DE
Alexander H. Yang - Campbell CA, US
Liv E. Jensen - Palo Alto CA, US
Marshall Porter - Santa Clara CA, US
Charlotte Yang - San Jose CA, US
Corissa Harris - Santa Clara CA, US
Matt Dipetro - Gilroy CA, US

Assignee:

Genia Technologies, Inc. - Santa Clara CA

International Classification:

C12Q 1/6869
C12N 9/12

Abstract:

A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.

US Patent:

20180362594, Dec 20, 2018

Filed:

Sep 20, 2016

Appl. No.:

15/762092

Inventors:

- Santa Clara CA, US
Tim CRAIG - Mountain View CA, US
Alexander YANG - Mountain View CA, US
Liv JENSEN - Mountain View CA, US
Charlotte YANG - Mountain View CA, US
Corissa HARRIS - Mountain View CA, US

Assignee:

GENIA TECHNOLOGIES, INC. - Santa Clara CA

International Classification:

C07K 14/245
C12N 9/12
C12Q 1/6874

Abstract:

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

US Patent:

20170088890, Mar 30, 2017

Filed:

Sep 23, 2016

Appl. No.:

15/274770

Inventors:

- Santa Clara CA, US
Cynthia Ann CECH - Newcastle WA, US
Michael DORWART - Mtn. View CA, US
Liv Elisabeth JENSEN - Palo Alto CA, US
Marshall Winston PORTER - Santa Clara CA, US
Alexander Hyun-min YANG - Campbell CA, US

International Classification:

C12Q 1/68
C07K 14/31

Abstract:

Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.