Amit C J Vas

US Patent:

20200270644, Aug 27, 2020

Filed:

Dec 16, 2016

Appl. No.:

16/062771

Inventors:

- Wayzata MN, US
Ana Negrete-Raymond - Chanhassen MN, US
Brian J. Rush - Minneapolis MN, US
Amit Vas - Minneapolis MN, US
Jon Veldhouse - Plymouth MN, US

International Classification:

C12P 7/10
C12N 1/16
C12N 15/52
C07K 14/39

Abstract:

Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO:44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

US Patent:

20200172855, Jun 4, 2020

Filed:

Aug 2, 2018

Appl. No.:

16/638251

Inventors:

- Wayzatz MN, US
Holly JESSEN - Chanhassen MN, US
Erin Kathleen MARASCO - Excelsior MN, US
Thomas William MCCMULLIN - Minnetonka MN, US
Ana NEGRETE-RAYMOND - Chanhassen MN, US
Amit VAS - Minneapolis MN, US

Assignee:

CARGILL, INCORPORATED - Wayzatz MN

International Classification:

C12N 1/16
C07K 14/39

Abstract:

Less-than-diploid cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

US Patent:

20200048623, Feb 13, 2020

Filed:

Jul 15, 2019

Appl. No.:

16/511319

Inventors:

- Wayzata MN, US
Ana NEGRETE-RAYMOND - Chanhassen MN, US
Jon VELDHOUSE - Plymouth MN, US
Amit VAS - Minneapolis MN, US

Assignee:

CARGILL, INCORPORATED - Wayzata MN

International Classification:

C12N 9/34
C07K 14/395
C12P 19/14
C12N 9/16

Abstract:

The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a mating factor alpha 2 (Sc MFα2) or repressible acid phosphatase (Sc PHO5) secretion signal.

US Patent:

20190376071, Dec 12, 2019

Filed:

Jun 12, 2019

Appl. No.:

16/439244

Inventors:

- Wayzata MN, US
Ana Negrete-Raymond - Chanhassen MN, US
Jon Veldhouse - Plymouth MN, US
Amit Vas - Minneapolis MN, US

Assignee:

CARGILL, INCORPORATED - Wayzata MN

International Classification:

C12N 15/81
C12P 7/10

Abstract:

Genetically engineered yeast with a heterologous glucoamylase and fermentation methods are described. The engineered yeast can have multiple exogenous nucleic acid sequences which each have a different sequence, but that encode the same or a similar glucoamylase protein that is heterologous to the yeast. The engineered yeast exhibit desirable bioproduct production profiles during a fermentation process. A fermentation medium with a starch material can be fermented with the engineered yeast to provide high ethanol titers, low glycerol titers, or both.

US Patent:

20190345471, Nov 14, 2019

Filed:

Aug 4, 2017

Appl. No.:

16/323458

Inventors:

- Wayzata MN, US
Gregory Michael POYNTER - St. Paul MN, US
Amit VAS - Minneapolis MN, US

Assignee:

CARGILL, INCORPORATED - Wayzata MN

International Classification:

C12N 9/34
C12P 7/06
C12P 19/14
C12P 17/04
C12P 13/04

Abstract:

The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An C aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).

US Patent:

20180080014, Mar 22, 2018

Filed:

Feb 5, 2016

Appl. No.:

15/548812

Inventors:

- WAYZATA MN, US
Ana NEGRETE-RAYMOND - Chanhassen MN, US
Jon VELDHOUSE - Plymouth MN, US
Amit VAS - Minneapolis MN, US

International Classification:

C12N 9/34
C07K 14/395
C12N 9/16
C12P 19/14

Abstract:

The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a mating factor alpha 2 (Sc MFα2) or repressible acid phosphatase (Sc PHO5) secretion signal.