Amy Sly Liu

US Patent:

6365410, Apr 2, 2002

Filed:

May 19, 1999

Appl. No.:

09/314847

Inventors:

Volker Schellenberger - Palo Alto CA
Amy D. Liu - Mountain View CA
Olga V. Selifonova - Los Altos CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N 1574

US Classification:

435488, 435440, 4352523, 43525233, 435243, 4353201

Abstract:

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

US Patent:

6436632, Aug 20, 2002

Filed:

Oct 4, 2001

Appl. No.:

09/970578

Inventors:

Volker Schellenberger - Palo Alto CA
Amy Deming Liu - Mountain View CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12Q 100

US Classification:

435 4, 4352831, 435 29, 435 30, 422 50, 422 681

Abstract:

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study.

US Patent:

6706503, Mar 16, 2004

Filed:

Oct 23, 2001

Appl. No.:

10/037677

Inventors:

Volker Schellenberger - Palo Alto CA
Amy D. Liu - Mountain View CA
Olga V. Selifonova - Los Altos CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 104

US Classification:

435170, 4352523, 435440, 435441, 435233, 435234, 435195, 435183, 4351738, 435471, 435479, 435481, 435482, 435 691

Abstract:

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

US Patent:

7125695, Oct 24, 2006

Filed:

Nov 20, 2003

Appl. No.:

10/719571

Inventors:

Volker Schellenberger - Palo Alto CA, US
Amy D. Liu - Mountain View CA, US
Olga V. Selifonova - Los Altos CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 1/04
C12P 21/06
C12N 15/00
C12N 9/90
C12N 9/00
C12N 15/74
C12N 1/20
C12N 15/01

US Classification:

435170, 435 691, 435440, 435441, 435233, 435234, 4352523, 435183, 4351738, 435195, 435479, 435481, 435482, 435471

Abstract:

The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.

US Patent:

7666360, Feb 23, 2010

Filed:

Aug 20, 2002

Appl. No.:

10/223893

Inventors:

Volker Schellenberger - Palo Alto CA, US
Amy Deming Liu - Mountain View CA, US

Assignee:

BioTrove, Inc. - Woburn MA

International Classification:

B01L 3/00

US Classification:

422102, 422104

Abstract:

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study.

US Patent:

8236542, Aug 7, 2012

Filed:

Jun 3, 2009

Appl. No.:

12/477887

Inventors:

Thijs Kaper - Palo Alto CA, US
Bradley R Kelemen - Menlo Park CA, US
Amy D. Liu - Sunnyvale CA, US

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12N 9/14
C12P 19/00
C12P 7/06

US Classification:

435195, 435 72, 435161

Abstract:

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

US Patent:

20050059074, Mar 17, 2005

Filed:

Oct 20, 2004

Appl. No.:

10/969104

Inventors:

Volker Schellenberger - Palo Alto CA, US
Amy Liu - Mountain View CA, US

International Classification:

C12Q001/68
G01N033/53
C12N015/85

US Classification:

435006000, 435007100, 435455000

Abstract:

A spatially fixed library of mutated cells and a method for creating such a library. The library is created by creating a set of cells having a genetic diversity, causing the cells to multiply and express phenotypes, preparing a solution containing the cells diluted in a medium, populating a plurality of through-holes of a testing plate with the solution such that surface tension holds the solution in respective through-holes, and analyzing the phenotypes of the cells in the solution on a hole-by-hole basis. Cells expressing specified phenotypes may then be retrieved.

US Patent:

20060183171, Aug 17, 2006

Filed:

Mar 17, 2006

Appl. No.:

11/378735

Inventors:

Volker Schellenberger - Palo Alto CA, US
Amy Liu - Mountain View CA, US

International Classification:

G01N 33/53

US Classification:

435007720

Abstract:

A high throughput screening method. At least one liquid sample is provided that includes an analyte that directly or indirectly produces a detectable characteristic in one or more first assays. A plurality of through holes is filled with portions of the liquid sample in a testing assembly that has a pair of opposing surfaces, each through hole extending from one of the opposing surfaces to the other of the opposing surfaces, with the through holes arranged in group. Surface tension holds the respective portions in the respective plurality of through holes. A detection is made of which of the through holes contains a liquid sample portion that includes said analyte. The detectable characteristic may be produced in various assays such as an absorbance transcription assay, a fluorescent transcription assay, a fluorescent secreted enzyme assay, or a microorganism screening assay.