Andreas Braun

US Patent:

6428955, Aug 6, 2002

Filed:

Nov 6, 1996

Appl. No.:

08/744481

Inventors:

Kai Tang - Brighton MA
Carsten W. Siegert - Hamburg, DE
Daniel P. Little - San Diego CA
Andreas Braun - San Diego CA
Brigitte Darnhofer-Demar - Hamburg, DE
Christian Jurinke - Hamburg, DE
Dirk Van den Boom - Dreieich, DE

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

The invention provides fast and highly accurate mass spectrometer based processes for detecting a particular nucleic acid sequence in a biological sample. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.

US Patent:

6602662, Aug 5, 2003

Filed:

Nov 28, 2000

Appl. No.:

09/724877

Inventors:

Daniel P. Little - Boston MA
Andreas Braun - San Diego CA

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911

Abstract:

Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity. One aspect provides a process for determining whether a target nucleotide is present in a nucleic acid molecule including hybridizing a nucleic acid molecule with a primer oligonucleotide; contacting the hybridized primer with deoxyribonucleoside triphosphates, chain terminating nucleotides and a DNA polymerase, whereby the hybridized primer is extended until a chain terminating nucleotide is incorporated, producing an extended primer, and determining the molecular mass of the extended primer, thereby determining whether the target nucleotide is present in a nucleic acid molecule.

US Patent:

6958214, Oct 25, 2005

Filed:

Apr 12, 2001

Appl. No.:

09/834700

Inventors:

Andreas Braun - San Diego CA, US

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C07H021/04
C12Q001/68

US Classification:

435 6, 435 911, 435 912, 4353201, 435325, 435 691, 536 231, 536 243, 536 234, 536 2431, 536 2433

Abstract:

Polymorphic A-kinase anchor proteins (AKAPs) and nucleic acids encoding the proteins are provided herein. Methods of detecting polymorphic AKAPs and nucleic acids encoding the AKAPs, and kits for use in the detection methods are also provided. Further provided herein are methods of identifying subjects having or at risk of developing disorders of signal transduction. Methods of determining susceptibility to morbidity and/or increased or early mortality are also provided.

US Patent:

7198893, Apr 3, 2007

Filed:

Oct 10, 2000

Appl. No.:

09/686148

Inventors:

Hubert Köster - Figino, CH
Andreas Braun - San Diego CA, US
Dirk van den Boom - La Jolla CA, US
Christian Jurinke - La Jolla CA, US
Andreas Ruppert - Hamburg, DE
Franz Hillenkamp - Münster, DE
Daniel P. Little - Boston MA, US

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911, 435 912

Abstract:

Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.

US Patent:

7332275, Feb 19, 2008

Filed:

Oct 15, 2002

Appl. No.:

10/272665

Inventors:

Andreas Braun - San Diego CA, US
Dirk Van Den Boom - Hamburg, DE
Christian Jurinke - Hamburg, DE

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 1/68
G01N 24/00
G06F 19/00

US Classification:

435 6, 702 19, 702 23, 436173

Abstract:

A method for detecting methylated nucleotides within a nucleic acid sample is provided. The method includes the steps of splitting a nucleic acid sample into separate reaction vessels; contacting nucleic acid in one reaction vessel with bisulfite; amplifying the nucleic acid in each reaction vessel; cleaving the nucleic acids in each reaction vessel to produce fragments thereof; and obtaining a mass spectrum of the resulting fragments from one reaction vessel and another mass spectrum of the resulting fragments from another reaction vessel.

US Patent:

7432342, Oct 7, 2008

Filed:

May 1, 2003

Appl. No.:

10/428254

Inventors:

Andreas Braun - San Diego CA, US
Charles R. Cantor - Del Mar CA, US
Stefan M. Kammerer - San Diego CA, US
Susan Taylor - Del Mar CA, US
Charles Cook - Mendham NJ, US
Gary Olson - Moutainside NJ, US
Christopher Self - West Caldwell NJ, US

Assignee:

Sequenom, Inc. - San Diego CA
The Regents of the University of Califonia - Oakland CA

International Classification:

C07K 7/08
C07K 14/00

US Classification:

530324, 530325, 530326

Abstract:

A-kinase anchor protein (AKAPs) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein. Also provided are transgenic animals, cells comprising transgenes and various methods employing such peptides.

US Patent:

7501251, Mar 10, 2009

Filed:

Oct 2, 2006

Appl. No.:

11/541871

Inventors:

Hubert Köster - Figino, CH
Andreas Braun - San Diego CA, US
Dirk van den Boom - La Jolla CA, US
Christian Jurinke - La Jolla CA, US
Andreas Ruppert - Hamburg, DE
Franz Hillenkamp - Munster, DE

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

Provided are methods for detecting a target nucleic acid in a biological sample using RNA amplification. In an embodiment, provided is a method that comprises (a) amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; (b) synthesizing RNA using an RNA polymerase that recognizes the promoter; and (c) detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.

US Patent:

7510835, Mar 31, 2009

Filed:

Nov 25, 2003

Appl. No.:

10/723681

Inventors:

Richard B. Roth - La Jolla CA, US
Matthew Roberts Nelson - San Marcos CA, US
Stefan M. Kammerer - San Diego CA, US
Andreas Braun - San Diego CA, US
Rikard Reneland - San Diego CA, US

Assignee:

Sequenom, Inc. - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04
C07H 21/02

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243

Abstract:

Provided herein are methods for identifying risk of breast cancer in a subject and/or a subject at risk of breast cancer, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating breast cancer, and therapeutic methods for treating breast cancer in a subject. These embodiments are based upon an analysis of polymorphic variations in nucleotide sequences within the human genome.