Anton M Jetten

US Patent:

54200334, May 30, 1995

Filed:

Jun 21, 1989

Appl. No.:

7/368725

Inventors:

Anton M. Jetten - Durham NC

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12Q 102
C12N 506
C12N 1506

US Classification:

4352402

Abstract:

An airway epithelial cell line (CF/T43) was developed by infecting cultured cystic fibrosis (CF) airway epithelial cells with the pZIPneoSV(X)1/SV40T retrovirus and selecting for Genetian (G418) resistance and ion transport properties. The distinctive chloride secretory phenotype of CF/T43 [an apical membrane chloride permeability (P. sub. cl -) activated by calcium-mediated, but not by adenosine 3',5'-monophosphate (cAMP)-dependent agonists] was not perturbed by SV40T-induced cell transformation. Epithelial cell lines generated from CF cells with the SV40T gene can be used to test candidate CF genes, to evaluate the molecular mechanisms responsible for the CF phenotype and to test putative therapeutic CF drugs.