Aparna D Vedula

Aparna Vedula Santa Clara, CA

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Work:

SG Biofuels

2011 to 2000
Research Associate Molecular Response
San Diego, CA
Jul 2010 to Jan 2011
Research Intern San Diego State University
San Diego, CA
2009 to 2010
Lab manager San Diego State University
San Diego, CA
2007 to 2010
Graduate Teaching Assistant San Diego State University
San Diego, CA
Graduate Level Research: Symposiums and conferences
San Diego, CA
POSTERS/PRESENTATIONS

Education:

San Diego State University
San Diego, CA
2010
Masters of Science in Cell and Molecular biology Jawaharlal Nehru Technological University
Hyderabad, Andhra Pradesh
May 2006
Bachelor of Technology in Biotechnology

Skills:

TECHNICAL SKILLS Molecular Biology: Polymerase Chain Reaction(PCR)Assays with various enzymes, Primer design, Gel electrophoresis and imaging DNA: High-throughput isolation using different protocols, quantification using various systems RNA: Isolation and optimization using different protocols from variety of tissues High Resolution Melt (HRM) technology- Assay design, experiment setup and analysis Automation: Qiagility instrument-Programming for complex reaction setup, optimization and trouble shooting Sequencing: Pyrosequencing with Pyromark 24- Assay design and setup; Run setup and analysis of results NGS: Ion Proton sequencing platform Quantitative PCR(qPCR) Gene cloning, transformations, minipreps to isolate plasmid, ligations, restriction digests Bioinformatics Sequence analysis: CLC genomics, Bioedit, DNAstar package, BLAST and multiple sequence alignment tools Phylogenetic analysis: MEGA package, Mr Bayes, PAML, Diverge2 Genomic tools: NCBI genomes, UCSC-Broad institute, JGI, ENSEMBL, TAIR, GEO, GeneSpring Programming skills: Python, C

Aparna Vedula

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