Arkansas State University - Jonesboro, Arkansas, USA since May 2009
Associate Professor in Metabolic Engineering
Arkansas State University Aug 2005 - May 2009
Assistant Professor in Metabolic Engineering
Virginia Tech - Blacksburg, VA Apr 2002 - Aug 2005
Post-Doctoral Research Associate
CEIB/Universidad Autonoma del Estado de Morelos - Cuernavaca, Morelos, Mexico Apr 1998 - Mar 2002
Assistant Professor
Education:
Universidad Nacional Autónoma de México 1995 - 1997
Doctor of Philosophy (PhD), Biotechnology
Universidad Nacional Autónoma de México 1992 - 1995
Master's degree, Biotechnology
Universidad Autonoma Metropolitana - Iztapalapa 1987 - 1991
Bachelor's degree, Biochemical Engineering
Skills:
Biochemistry Protein Expression Protein Purification Molecular Cloning Science Molecular Biology Dna Pcr Teaching Enzyme Assays Metabolic Engineering Genetics Tissue Culture Sds Page Recombinant Dna Technology Higher Education Gel Electrophoresis Western Blotting Cell Biology Biotechnology Genomics Microscopy Protein Chemistry Grant Writing Plant Tissue Culture Dna Extraction Fluorescence Microscopy Enzyme Kinetics Rna Isolation Elisa Transgenic Plants Community Outreach Plant Transformation Uv/Vis Bacterial Transformation Pcr Primer Design Antioxidants Phytoremediation Phenomics Cross Cultural Communication Skills Multi Cultural Team Leadership Cross Functional Team Leadership Cross Functional Team Building Cross Functional Coordination Intercultural Awareness Bilingual Communications
Interests:
Antioxidants Plant Biotechnology Phytoremediation Medicinal Plants Vitamin C Plant Biochemistry
Methods are provided for increasing plant growth rate, biomass and tolerance to stress by genetically engineering plants to contain and express a gene of the ascorbic acid synthesis-cell wall synthesis network (e.g. GlcUA reductase, GLOase or MIOX). Transgenic plants that are genetically engineered in such a manner are also provided.
Methods And Compositions For Enhancing Polypeptide Production
Methods of increasing production, stability or activity of a target polypeptide are provided herein. The method includes increasing the level of an antioxidant in a cell comprising a polynucleotide encoding the target polypeptide. Also provided are cells and transgenic organisms produced using the methods described herein. Methods of treating a subject with a condition treatable by administration of the target polypeptide are also disclosed. Finally methods and compositions for transiently increasing antioxidants in plant cells are provided.
Craig Nessler - Blacksburg VA, US Argelia Lorence - Blacksburg VA, US Boris Chevone - Blacksburg VA, US Pedro Mendes - Blacksburg VA, US
International Classification:
A01H 1/00 C12N 15/82 A01H 5/00
US Classification:
800278000, 800317300
Abstract:
Methods for increasing the vitamin C content of plants are provided by transforming the plants with genes encoding a novel vitamin C biosynthetic pathway. Vitamin C production is increased in the resulting transgenic plants, providing, for example, higher nutritional value and longer shelf-life of produce. Further, the leaves of air-cured varieties of tobacco transformed in this manner contain lower levels of highly carcinogenic tobacco specific nitrosamines (TSNAs).