Arlene E Nunez

US Patent:

20120034606, Feb 9, 2012

Filed:

Aug 3, 2011

Appl. No.:

13/197758

Inventors:

Robert TEBBS - Livermore CA, US
Craig Cummings - Pacifica CA, US
Arlene Nunez - San Jose CA, US
Priya Balachandran - Foster City CA, US
Payman Fatemi - Pleasanton CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 612

Abstract:

Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of , subspecies , serovar () in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect without cross-reacting with C or other closely related species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.