Armando E Giuliano

US Patent:

20100068743, Mar 18, 2010

Filed:

Sep 2, 2009

Appl. No.:

12/584357

Inventors:

Armando E. Giuliano - Los Angeles CA, US
Dave S. Hoon - Los Angeles CA, US
Hideki Ishihara - Miki-shi, JP

International Classification:

C12Q 1/48

US Classification:

435 15

Abstract:

A method predicts a response of a cancer patient to anticancer drug based on a cell cycle profile score obtained by analyzing a malignant tumor of the cancer patient, wherein the method can predict a disease outcome including a complete response, a partial response, stable disease, no response, and time to progression of disease as patient's response to anticancer drug therapy.

US Patent:

20200002671, Jan 2, 2020

Filed:

Jan 25, 2018

Appl. No.:

16/480778

Inventors:

- Los Angeles CA, US
Xiaojiang CUI - Arcadia CA, US
Dhruv SAREEN - Porter Ranch CA, US
Armando E. GIULIANO - Los Angeles CA, US

Assignee:

Cedars-Sinai Medical Center - Los Angeles CA

International Classification:

C12N 5/071
A61K 35/55
C12N 5/074

Abstract:

Human induced pluripotent stem cells (iPSCs) can give rise to multiple cell types and hold great promise in regenerative medicine and disease modeling applications. The Inventors herein developed a reliable two-step protocol to generate human mammary-like organoids from iPSCs. Non-neural ectoderm cell-containing spheres, referred to as mEBs, were first differentiated and enriched from iPSCs using MammoCult medium. Gene expression profile analysis suggested that mammary gland function-associated signaling pathways were hallmarks of 10-d differentiated mEBs. The Inventors generated mammary-like organoids from 10-d mEBs using 3D floating mixed gel culture and a three-stage differentiation procedure. These organoids expressed common breast tissue, luminal, and basal markers, including estrogen receptor, and could be induced to produce milk protein. These results demonstrate that human iPSCs can be directed in vitro toward mammary lineage differentiation.