Benjamin David Grant

US Patent:

20210370290, Dec 2, 2021

Filed:

May 29, 2020

Appl. No.:

16/886934

Inventors:

- Bellevue WA, US
Joshua Davis Bishop - Seattle WA, US
David Michael Cate - Bellevue WA, US
Benjamin David Grant - Bellevue WA, US
Toan Huynh - Seattle WA, US
Damian Madan - Issaquah WA, US
Kevin Paul Flood Nichols - Issaquah WA, US
Bernhard Hans Weigl - Seattle WA, US

International Classification:

B01L 3/00
C12M 1/00
C12M 1/12

Abstract:

Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

US Patent:

20210055282, Feb 25, 2021

Filed:

Nov 6, 2020

Appl. No.:

17/091501

Inventors:

- Schenectady NY, US
- Bellevue WA, US
Cathryn Ellen Olsen - Wilton NY, US
John Richard Nelson - Clifton NY, US
David Roger Moore - Rexford NY, US
Paul Michael Smigelski - Glenville NY, US
John Thomas Connelly - Seattle WA, US
Benjamin David Grant - Bellevue WA, US
Bernhard Hans Weigl - Seattle WA, US

International Classification:

G01N 33/493
C07K 1/22
B01D 15/08
G01N 33/50
G01N 33/569
B01D 63/02
A61B 10/00
C12M 1/26
G01N 33/558

Abstract:

A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

US Patent:

20190383807, Dec 19, 2019

Filed:

Jun 13, 2018

Appl. No.:

16/006984

Inventors:

- Bellevue WA, US
Jeffrey L. Dantzler - Seattle WA, US
Veronika A. Glukhova - Seattle WA, US
Benjamin David Grant - Bellevue WA, US
Stephen Paul Harston - Bothell WA, US
Vedrana Ivezic - Seattle WA, US
Bernhard Hans Weigl - Seattle WA, US
Ozgur Emek Yildirim - Bellevue WA, US

International Classification:

G01N 33/543
G01N 1/40
B01L 3/00
G01N 33/569

Abstract:

Methods and systems for capture concentration of analytes using lectins and other capture ligands are described. For example, stationary phase media functionalized with lectins are used for capture concentration and cleaning of TB lipoarabinomannan (TB LAM) prior to assay on a lateral flow assay (LFA) device, and filtration devices suitable for particulate or bulk capture media are described. Size-exclusion filtration is used to separate particles with captured analyte during washing and concentration steps. Captured analyte can be eluted from stationary phase media prior to application to a LFA or eluted directly onto a customized LFA device that includes a size-selective filter. In various aspects, a size-selective filter on a LFA is used to transfer particulate capture media on a LFA device.

US Patent:

20190187126, Jun 20, 2019

Filed:

Dec 20, 2017

Appl. No.:

15/848302

Inventors:

- Schenectady NY, US
- Bellevue WA, US
Cathryn Ellen OLSEN - Wilton NY, US
John Richard NELSON - Clifton Park NY, US
David Roger MOORE - Rexford NY, US
John Thomas CONNELLY - Seattle WA, US
Benjamin David GRANT - Bellevue WA, US
Bernhard Hans WEIGL - Seattle WA, US

International Classification:

G01N 33/493
C07K 1/22
B01D 15/08
G01N 33/50
G01N 33/569
B01D 63/02
C12M 1/26
A61B 10/00

Abstract:

A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

US Patent:

20180290080, Oct 11, 2018

Filed:

Mar 29, 2018

Appl. No.:

15/939756

Inventors:

- Bellevue WA, US
John Thomas Connelly - Seattle WA, US
Benjamin David Grant - Bellevue WA, US
Stephen Paul Harston - Bothell WA, US
Isaac Santos - Seatac WA, US
Benjamin K. Wilson - Snoqualmie WA, US
Ozgur Emek Yildirim - Bellevue WA, US

International Classification:

B01D 29/90
G01N 1/40
B01L 3/02
B01L 9/00
B01L 3/00
B01D 29/05
B05C 17/01

Abstract:

A device for application of even pressure on a plunger head includes: a center rod with a pressure plate affixed at a first end and a back plate positioned adjacent to a second end; a positioning spring adjacent to the pressure plate at a surface distal to the center rod; a positioning plate adjacent to an end of the positioning spring distal to the pressure plate; a housing including an aperture at a position adjacent to a distal surface of the positioning plate; a main spring surrounding the center rod between the back plate and the pressure plate; and a mechanism positioned to move the center rod between the pressure plate and the back plate, the mechanism positioned to compress the main spring between the pressure plate and the back plate in response to force applied by a user.