Benjamin M Gray

US Patent:

20200308597, Oct 1, 2020

Filed:

Jun 14, 2017

Appl. No.:

16/311377

Inventors:

- Research Triangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

Benson Hill Biosystems, Inc. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 9/12

Abstract:

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ADP-glucose pyrophosphorylase small subunit (AGPaseSS) proteins, polypeptides encompassing AGPaseSS proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

US Patent:

20190338296, Nov 7, 2019

Filed:

Apr 24, 2019

Appl. No.:

16/393062

Inventors:

- Research Trangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

BENSON HILL BIOSYSTEMS, INC. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 15/10
C12N 9/22

Abstract:

Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

US Patent:

20190071688, Mar 7, 2019

Filed:

Sep 19, 2018

Appl. No.:

16/135724

Inventors:

- Research Triangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

BENSON HILL BIOSYSTEMS, INC. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 9/22
C12N 15/10
C12N 15/62

Abstract:

Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cpf1 or Csm1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cpf1 or Csm1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

US Patent:

20190048357, Feb 14, 2019

Filed:

Aug 8, 2018

Appl. No.:

16/058718

Inventors:

- Research Triangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

BENSON HILL BIOSYSTEMS, INC. - Research Triangle Park NC

International Classification:

C12N 15/82

Abstract:

Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

US Patent:

20180148735, May 31, 2018

Filed:

Nov 8, 2017

Appl. No.:

15/806890

Inventors:

- Research Triangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

BENSON HILL BIOSYSTEMS, INC. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 9/22
C12N 15/10
C12N 15/62

Abstract:

Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cpf1 or Csm1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cpf1 or Csm1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

US Patent:

20170233756, Aug 17, 2017

Filed:

Feb 14, 2017

Appl. No.:

15/432109

Inventors:

- Research Triangle Park NC, US
Benjamin Neil Gray - Chapel Hill NC, US

Assignee:

BENSON HILL BIOSYSTEMS, INC. - Research Triangle Park NC

International Classification:

C12N 15/82
C12N 9/22
C12N 15/62

Abstract:

Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cpf1 or Csm1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cpf1 or Csm1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci.

US Patent:

20170198298, Jul 13, 2017

Filed:

Jul 8, 2015

Appl. No.:

15/324501

Inventors:

- Research Triangle Park NC, US
Benjamin N. Gray - Chapel Hill NC, US
Todd C. Mockler - St. Louis MO, US
Lin Wang - Grover MO, US

Assignee:

Benson Hill Biosystems, Inc. - Research Triangle Park NC

International Classification:

C12N 15/82
C07K 14/415

Abstract:

Compositions and methods for improving plant growth are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing transcription factors (TFs) whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.