Bernhardt L Trout from Lexington, MA, age 57

Us Patents

Novel Antibody Molecules And Nucleic Acids Binding To Fungal Stress Protein Hsp90
view source
US Patent:

20100113355, May 6, 2010
Filed:

Apr 24, 2006
Appl. No.:

12/597229
Inventors:

Naresh Chennamsetty - Cambridge MA, US
Stefan Ewert - Allschwil, CH
Bernard Helk - Riehen, CH
Bernhardt Trout - Lexington MA, US
Philipp Wechner - Schwaz, AT
International Classification:

A61K 38/16
C07K 14/00
C07H 21/04
C12N 15/63
C12N 1/21
C12P 21/02
A61P 35/00
US Classification:

514 12, 530350, 536 231, 4353201, 4352523, 435 691
Abstract:

An scFv peptide comprising a Vdomain and a Vdomain linked by an amino acid spacer is disclosed. The Vdomain comprises a sequence with at least 80% sequence identity to the sequence of SEQ ID NO. 64. The Vdomain comprises a sequence with at least 80% sequence identity to the sequence of SEQ ID NO. 66. The scFv peptide comprises an additional feature selected from (a) to (e) and combinations thereof. (a) A substitution or deletion of an amino acid in the Vdomain at a position corresponding to that selected from the group consisting of: I, H, N, Cand combinations thereof. (b) A substitution or deletion of an amino acid in the Vdomain at a position corresponding to that selected from the group consisting of: V, V, F, F, A, Nand combinations thereof. (c) The amino acid spacer comprises the sequence (GGGGS)wherein n is between 4 and 6. (d) The Vdomain further comprises an N-terminal pelB signal sequence comprising the sequence of SEQ ID NO. 68 or a sequence having at least 80% sequence identity thereto. (e) The Vdomain is located at the N-terminal end of the Vdomain. (f) The substitution of the cysteine residue in the Vdomain corresponding to position 28 with a serine residue.

Methods To Identify Macromolecule Binding And Aggregation Prone Regions In Proteins And Uses Therefor
view source
US Patent:

20110257104, Oct 20, 2011
Filed:

Jun 19, 2009
Appl. No.:

13/000353
Inventors:

Naresh Chennamsetty - Cambridge MA, US
Bernhard Helk - Basel, CH
Bernhardt Trout - Cambridge MA, US
Veysel Kayser - Cambridge MA, US
Vladimir Voynov - Cambridge MA, US
Assignee:

MASSACHUSETTS INSTITUTE OF TECHNOLOGY - Cambridge MA
NOVARTIS AG - Basel
International Classification:

A61K 38/16
A61P 37/00
G06F 19/00
C07K 14/00
US Classification:

514 212, 530402, 702 19
Abstract:

The present invention provides methods and computational tools based, at least in part, on computer simulations that identify macromolecule binding regions and aggregation prone regions of a protein. Substitutions may then be made in these aggregation prone regions to engineer proteins with enhanced stability and/or a reduced propensity for aggregation. Similarly, substitutions may then be made in these macromolecule binding regions to engineer proteins with altered binding affinity for the macromolecule.

Immunoglobulins With Reduced Aggregation
view source
US Patent:

20120003211, Jan 5, 2012
Filed:

Jun 19, 2009
Appl. No.:

13/000351
Inventors:

Naresh Chennamsetty - Cambridge MA, US
Bernhard Helk - Basel, CH
Veysel Kayser - Cambridge MA, US
Bernhardt Trout - Cambridge MA, US
Vladimir Voynov - Cambridge MA, US
Assignee:

MASSACHUSETTS INSTITUTE OF TECHNOLOGY - Cambridge MA
NOVARTIS AG - Base;
International Classification:

A61K 39/395
C12N 5/10
C12N 15/63
C12P 21/00
C07K 16/00
C07H 21/00
US Classification:

4241331, 5303873, 536 2353, 4353201, 435 696, 435328
Abstract:

The present disclosure relates to immunoglobulins with reduced aggregation and compositions, methods of generating such immunoglobulins with computational tools and methods of using such immunoglobulins particularly in the treatment and prevention of disease.

Compositions, Methods, And Systems Relating To Controlled Crystallization And/Or Nucleation Of Molecular Species
view source
US Patent:

20120076860, Mar 29, 2012
Filed:

Aug 23, 2011
Appl. No.:

13/216018
Inventors:

Bernhardt Levy Trout - Lexington MA, US
Patrick S. Doyle - Boston MA, US
Trevor Alan Hatton - Sudbury MA, US
Allan Stuart Myerson - Boston MA, US
Ying Diao - Palo Alto CA, US
Matthew E. Helgeson - Somerville MA, US
Assignee:

Massachusetts Institute of Technology - Cambridge MA
International Classification:

A61K 9/14
C30B 19/12
US Classification:

424489, 117 64
Abstract:

The present invention generally relates to compositions, methods, and systems relating to controlled crystallization and/or nucleation of a molecular species. In some embodiments, the crystallization and/or nucleation of the molecular species may be controlled by tuning the surface chemistry and/or the morphology of a crystallization substrate. In some embodiments, the molecular species is a small organic molecule (e.g., pharmaceutically active agent).

Methods For Identification Of Sites For Igg Conjugation
view source
US Patent:

20120148580, Jun 14, 2012
Filed:

Jun 4, 2010
Appl. No.:

13/375466
Inventors:

Naresh Chennamsetty - Cambridge MA, US
Bernhard Helk - Basel, CH
Voysel Kayser - Cambridge MA, US
Bernhardt Trout - Cambridge MA, US
Vladmir Voynov - Cambridge MA, US
Assignee:

NOVARTIS AG - Basel
International Classification:

A61K 39/395
C07K 16/00
C12N 9/96
G06G 7/60
C12N 15/85
C12P 21/00
C12N 5/10
C07K 19/00
C12N 15/62
US Classification:

4241331, 5303873, 5303913, 435188, 5303911, 5303917, 536 234, 4353201, 435 696, 435328, 703 2
Abstract:

The present disclosure relates to immunoglobulins and immunoglobulin conjugates with reduced oligomerization and efficient labeling and compositions, methods of generating such immunoglobulins and immunoglobulin conjugates and methods of using such immunoglobulin conjugates particularly in the treatment and prevention of disease.

Layer Processing For Pharmaceuticals
view source
US Patent:

20120305174, Dec 6, 2012
Filed:

Apr 27, 2012
Appl. No.:

13/458222
Inventors:

Bernhardt Levy Trout - Lexington MA, US
Trevor Alan Hatton - Sudbury MA, US
Emily Chang - Edison NJ, US
James MB Evans - Sudbury MA, US
Salvatore Mascia - Boston MA, US
Won Kim - Gyeonggi-Do, KR
Ryan Richard Slaughter - Riverside CA, US
Yi Du - Union Township NJ, US
Himanshu Hemant Dhamankar - Cambridge MA, US
Keith M. Forward - Somerville MA, US
Gregory C. Rutledge - Newton MA, US
Mao Wang - Belmont MA, US
Allan Stuart Myerson - Boston MA, US
Blair Kathryn Brettmann - Cambridge MA, US
Nikhil Padhye - Cambridge MA, US
Jung-Hoon Chun - Sudbury MA, US
Assignee:

Massachusetts Institute of Technology - Cambridge MA
International Classification:

A61K 9/70
B29C 59/16
B29C 39/00
US Classification:

156227, 264299, 264447
Abstract:

Layer processing for pharmaceuticals, and related systems, methods, and articles are generally described. In some embodiments, ingestible pharmaceutical products (e.g., tablets) can be formed by processing one or more layers containing a pharmaceutically active composition. For example, at least one layer containing a pharmaceutically active composition can be manipulated (e.g., folded, rolled, stacked, etc.) such that the average thickness of the product formed by the manipulation is at least about two times the average thickness of the portions of the layer(s) used to form the product. In some embodiments, after the layer is manipulated, it can be processed (e.g., cut, coated, etc.) to form a final product such as, for example, a tablet.

Methods And Systems Relating To The Selection Of Substrates Comprising Crystalline Templates For The Controlled Crystallization Of Molecular Species
view source
US Patent:

20130118399, May 16, 2013
Filed:

Nov 15, 2012
Appl. No.:

13/677813
Inventors:

Massachusetts Institute of Technology - Cambridge MA, US
Bernhardt Levy Trout - Lexington MA, US
Allan Stuart Myerson - Boston MA, US
Assignee:

Massachusetts Institute of Technology - Cambridge MA
International Classification:

C30B 19/12
C09K 3/00
US Classification:

117 54, 423448, 5483391, 4234994, 53612313, 568852, 252 1
Abstract:

The present invention generally relates to methods and systems relating to the selection of substrates comprising crystalline templates for the controlled crystallization of molecular species. In some embodiments, the methods and systems allow for the controlled crystallization of a molecular species in a selected polymorphic form. In some embodiments, the molecular species is a small organic molecule (e.g., pharmaceutically active agent).

Solution Additives For The Attenuation Of Protein Aggregation
view source
US Patent:

20080247991, Oct 9, 2008
Filed:

Feb 28, 2005
Appl. No.:

10/590827
Inventors:

Bernhardt L. Trout - Cambridge MA, US
Daniel I.C. Wang - Newton MA, US
Brian M. Baynes - Somerville MA, US
International Classification:

A61K 38/21
C07H 3/02
C07C 31/18
C13K 13/00
C13K 1/00
C07C 279/14
C07D 207/16
C07D 239/28
C07C 233/47
G06F 19/00
C08G 65/02
C08G 65/34
C08G 69/26
C07K 14/505
C07K 14/555
C07K 1/107
C07K 16/00
C07K 14/62
A61K 38/02
A61K 39/395
A61K 38/28
A61K 38/22
US Classification:

424 854, 536 111, 568852, 53612313, 564240, 548535, 544335, 564153, 702 22, 702 19, 530410, 5303905, 530303, 514 2, 4241301, 4241331, 514 8, 530351, 530397, 528332, 528425, 528417, 526319
Abstract:

In part, the present invention relates to a compound or polymer comprising a non-protein-binding moiety and at least one protein-binding group. The present invention relates to a method of screening compounds or polymers for the property of inhibiting protein aggregation in solution, a method of preparing a compound or polymer having the property of protein aggregation inhibition in solution, a method of classifying a compound or polymer as either inhibitory of protein aggregation in solution or not inhibitory of protein aggregation in solution, and to a method of determining the preferential binding coefficient, Γ, of an additive in a protein solution. The present invention also relates to a method of suppressing or preventing aggregation of a protein in solution, a method of decreasing the toxicological risk associated with administering a protein to a mammal in need thereof, and a method of facilitating native folding of a recombinant protein in solution.

Name / Title

Company / Classification

Phones & Addresses

Bernhardt Trout
President

BERNANA, INC
Business Services at Non-Commercial Site

20 Partridge Rd, Lexington, MA 02420
PO Box 8326, Longboat Key, FL 34228
2043 Harbour Links Dr, Longboat Key, FL 34228

Bernhardt Trout

BAB ASSOCIATES OF SARASOTA, LTD

2043 Hbr Links Dr, Longboat Key, FL 34228
2043 Harbour Links Dr, Longboat Key, FL 34228

Resumes

Associate Professor Department Of Chemical Engineering

view source

Location:

Lexington, MA

Industry:

Higher Education

Work:

Hedgeness
Raymond F Baddour, Scd, Professor of Chemical Engineering, Mit and Member of the Advisory Board
Massachusetts Institute of Technology (Mit)
Associate Professor Department of Chemical Engineering

Education:

University of California, Berkeley 1991 - 1996
Massachusetts Institute of Technology 1986 - 1990

Skills:

Chemical Engineering
Formulation
R&D
Chemistry
Crystallization
Technology Transfer
Nanotechnology
Hplc
Life Sciences
Organic Chemistry
Materials Science
Science
Spectroscopy
Protein Chemistry
Drug Delivery
Characterization
Uv/Vis
Lifesciences
Uv/Vis Spectroscopy
Physical Chemistry
Nmr

Languages:

French
German
Italian