Bianca J Lam

US Patent:

20110300554, Dec 8, 2011

Filed:

Dec 31, 2009

Appl. No.:

13/138057

Inventors:

Gerald F. Joyce - Rancho Santa Fe CA, US
Bianca J. Lam - Encinitas CA, US

International Classification:

C12Q 1/68
C07H 21/02
G01N 33/53
C07H 21/00
G01N 33/50
G01N 33/68

US Classification:

435 619, 435 61, 436 94, 436501, 536 231

Abstract:

This invention provides nucleic acid molecules that catalyze their own replication and undergo exponential amplification at a constant temperature and in the absence of proteins or other biological components, such as those employed in other amplification reactions, e.g., proteins including DNA or RNA polymerase and a method of detect a selected molecule using said nucleic acid.

US Patent:

20160264950, Sep 15, 2016

Filed:

Aug 25, 2015

Appl. No.:

14/835609

Inventors:

- Carlsbad CA, US
Bianca Lam - Encinitas CA, US
Joanna Guo - San Diego CA, US
Lushen Li - San Diego CA, US

International Classification:

C12N 9/12
C12P 19/34

Abstract:

The present invention provides reversibly inactivated reverse transcriptase enzymes, particularly those having increased thermostability and/or thermoreactivity and compositions, methods and kits that include such enzymes, for the reverse transcription of nucleic acid molecules. Also provided are compositions and methods for the reactivation of reversibly inactivated reverse transcriptases. More particularly, the present invention relates to compositions and methods that can increase the speed of reactivation and stabilize reactivation of chemically modified reverse transcriptase enzymes prior to, or as part of a reverse transcription reaction. As compared to existing compositions and methods, the reversibly inactivated reverse transcriptase enzymes of the present invention provide for a significant reduction in non-specific reverse transcription from template nucleic acid molecules.