Bonnie Louise Bertolaet

US Patent:

20050208613, Sep 22, 2005

Filed:

Apr 29, 2005

Appl. No.:

11/118016

Inventors:

Yong Qin - Poway CA, US
Lin Yu - San Diego CA, US
Mark Hansen - San Diego CA, US
Eduard Sergienko - San Diego CA, US
Bonnie Bertolaet - San Diego CA, US
Daniel Sem - New Berlin WI, US

International Classification:

C12Q001/26
C07H019/207

US Classification:

435025000, 534727000, 536026240

Abstract:

The present invention provides compositions containing a common ligand linked to a detectable moiety and provides methods for the preparation of such compositions. The present invention also provides methods for screening candidate ligands for binding to a NAD binding receptor, which include contacting a receptor with a candidate ligand and a composition of the invention followed by evaluation of receptor binding. The screening method of the present invention has broad applicability and can be used to screen large numbers of a wide variety of ligands. The present invention further provides methods for detecting the binding activity of a putative receptor, which include combining the putative receptor with a composition of the invention and evaluating the level of detectable moiety. The invention also provides kits useful for detection of receptors having NAD binding activity and for screening of candidate ligands that bind to a NAD binding receptor.

US Patent:

20030228621, Dec 11, 2003

Filed:

Jul 2, 2002

Appl. No.:

10/189327

Inventors:

Yong Qin - Poway CA, US
Lin Yu - San Diego CA, US
Mark Hansen - San Diego CA, US
Eduard Sergienko - San Diego CA, US
Bonnie Bertolaet - San Diego CA, US
Daniel Sem - New Berlin WI, US

International Classification:

G01N033/53
C12Q001/26

US Classification:

435/007100, 435/025000

Abstract:

The present invention provides compositions containing a common ligand linked to a detectable moiety and provides methods for the preparation of such compositions. The present invention also provides methods for screening candidate ligands for binding to a NAD binding receptor, which include contacting a receptor with a candidate ligand and a composition of the invention followed by evaluation of receptor binding. The screening method of the present invention has broad applicability and can be used to screen large numbers of a wide variety of ligands. The present invention further provides methods for detecting the binding activity of a putative receptor, which include combining the putative receptor with a composition of the invention and evaluating the level of detectable moiety. The invention also provides kits useful for detection of receptors having NAD binding activity and for screening of candidate ligands that bind to a NAD binding receptor.