Bonnie L Hanson

US Patent:

20070218456, Sep 20, 2007

Filed:

Feb 8, 2007

Appl. No.:

11/672841

Inventors:

Bonnie Hanson - Madison WI, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12Q 1/00
C12Q 1/66
C12N 15/09

US Classification:

435004000, 435008000, 435325000, 435455000

Abstract:

The present invention provides cells and methods related to signaling receptors. The cells of the invention express the signaling receptors (e.g., in a constitutively active state). The cells are useful for analyzing the signaling receptors and their related pathways. The invention further provides methods for studying interactions of the signaling receptors and for small molecule screening, including high throughput methods. The invention further relates to expressing a signaling receptor (e.g., a GPCR) in a constitutively active state, even in the absence of the receptor's ligand. This allows for screening for inhibitors of the activated receptor's pathway without even knowing the ligand that activates the receptor, e.g., an orphan receptor. The invention further provides cell lines for expressing a signaling receptor in a constitutively active state. These cell lines are useful for high throughput screening assays of the invention.

US Patent:

20090317858, Dec 24, 2009

Filed:

Jun 17, 2009

Appl. No.:

12/486620

Inventors:

Bonnie Jean Hanson - Madison WI, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - 5791 Van Allen Way CA

International Classification:

C12Q 1/02
C12N 5/00
C12P 21/06
C12N 15/00

US Classification:

435 29, 435325, 435 691, 435455

Abstract:

The present invention provides cells and methods related to signaling receptors. The cells of the invention express the signaling receptors (e.g., in a constitutively active state). The cells are useful for analyzing the signaling receptors and their related pathways. The invention further provides methods for studying interactions of the signaling receptors and for small molecule screening, including high throughput methods. The invention further relates to expressing a signaling receptor (e.g., a GPCR) in a constitutively active state, even in the absence of the receptor's ligand. This allows for screening for inhibitors of the activated receptor's pathway without even knowing the ligand that activates the receptor, e.g., an orphan receptor. The invention further provides cell lines for expressing a signaling receptor in a constitutively active state. These cell lines are useful for high throughput screening assays of the invention.

US Patent:

20130210063, Aug 15, 2013

Filed:

Oct 13, 2011

Appl. No.:

13/879621

Inventors:

Bonnie Jean Hanson - Madison WI, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/02
G01N 33/50
C12Q 1/18

US Classification:

435 32, 435 29

Abstract:

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.

US Patent:

20180202996, Jul 19, 2018

Filed:

Mar 16, 2018

Appl. No.:

15/923502

Inventors:

- Carlsbad CA, US
Bonnie Jean HANSON - Madison WI, US

International Classification:

G01N 33/50
G01N 33/52
C12Q 1/02
C12Q 1/18

Abstract:

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.

US Patent:

20160054303, Feb 25, 2016

Filed:

Nov 3, 2015

Appl. No.:

14/931531

Inventors:

- Carlsbad CA, US
Bonnie Jean HANSON - Madison WI, US

International Classification:

G01N 33/50
C12Q 1/18

Abstract:

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.