Brian D Wall

US Patent:

20120101022, Apr 26, 2012

Filed:

Apr 2, 2010

Appl. No.:

13/262742

Inventors:

John Dayton Tovar - Baltimore MD, US
Stephen Robert Diegelmann - Baltimore MD, US
Brian D. Wall - Baltimore MD, US
Geeta Sophie Vadehra - Los Angeles CA, US

Assignee:

THE JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

A61K 38/02
C07K 7/06
B32B 3/00
C07K 2/00
C07K 1/04
B82Y 40/00
B82Y 5/00

US Classification:

514 11, 530323, 428220, 977788, 977925, 977840

Abstract:

The aqueous self-assembly of oligopeptide-flanked π-conjugated molecules into discrete one-dimensional nanostructures is described. Unique to these molecules is the fact that the π-conjugated unit has been directly embedded within the peptide backbone by way of a synthetic amino acid with π-functionality that is compatible with standard Fmoc-based peptide synthesis or by way of a diacid or other bis(electrophile) that can covalently cross-link peptide chains presented on a synthesis support. The peptide-based molecular designs enforce intimate π-π communication within the aggregates after charge-screening and self-assembly, making these nanostructures attractive for optical or electronic applications in biological environments. In other embodiments, a convenient method to incorporate π-electron units into peptides that assemble into amyloid-like supramolecular polymers is disclosed. Self-assembly manipulates these “electronic peptides” into delocalized sub-10 nm one dimensional (1-D) nanostructures under completely aqueous conditions.

US Patent:

20220348770, Nov 3, 2022

Filed:

Mar 21, 2022

Appl. No.:

17/699790

Inventors:

- Franklin Lakes NJ, US
Brian Wall - Poway CA, US
Jon Hollinger - Poway CA, US
Yongchao Liang - Irvine CA, US

International Classification:

C09B 69/10

Abstract:

Water-soluble dipyrromethene-based dyes are provided herein. In certain cases, the water-soluble dye is a dipyrromethene-boron dye. Embodiments of the subject water-soluble dipyrromethene-based dyes have a narrow absorption maxima in the yellow green range close to or at the 561 nm laser line, and are suitable for use in tandem dye systems. Embodiments of the subject dyes efficiently transfer energy to an acceptor chromophore while a second dipyrromethene-based dye of the same or different chemical structure is within energy-receiving proximity therewith. Also provided herein are tandem dyes that include the subject water-soluble dipyrromethene-based dyes, e.g., as donor chromophores. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

US Patent:

20200392346, Dec 17, 2020

Filed:

Aug 26, 2020

Appl. No.:

17/003591

Inventors:

- Franklin Lakes NJ, US
Yongchao Liang - Irvine CA, US
Brian Wall - Poway CA, US
David Moureau - San Marcos CA, US

International Classification:

C09B 69/10
G01N 33/58

Abstract:

Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.

US Patent:

20200002540, Jan 2, 2020

Filed:

Mar 28, 2019

Appl. No.:

16/368513

Inventors:

- Franklin Lakes NJ, US
Yongchao Liang - Irvine CA, US
Brian Wall - Poway CA, US
David Moureau - San Marcos CA, US

International Classification:

C09B 69/10
G01N 33/58

Abstract:

Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.

US Patent:

20190194467, Jun 27, 2019

Filed:

Dec 19, 2018

Appl. No.:

16/226381

Inventors:

- Franklin Lakes NJ, US
Jonathan Hollinger - La Jolla CA, US
Brian Wall - San Diego CA, US
David Moureau - San Marcos CA, US
Lucy Zhao - San Diego CA, US
Glenn P. Bartholomew - Escondido CA, US

International Classification:

C09B 69/10
C08G 61/10

Abstract:

Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.