Stanton Gelvin - West Lafayette IN, US Kirankumar Mysore - Ardmore OK, US Kan Wang - Ames IA, US Bronwyn Frame - Ames IA, US
International Classification:
A01H001/00 C12N015/82 C12N005/04
US Classification:
435/468000, 800/294000, 435/419000
Abstract:
Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.
Stanton B. Gelvin - West Lafayette IN, US Heiko Oltmanns - Dossenheim, DE Lan-Ying Lee - West Lafayette IN, US Bronwyn Frame - Ames IA, US Kan Wang - Ames IA, US
Assignee:
PURDUE RESEARCH FOUNDATION - West Lafayette IN IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. - Ames IA
International Classification:
C12N 15/82 C12N 1/21
US Classification:
800294, 4352522
Abstract:
Methods and compositions reduce copy number of transgenes and minimizing vector backbone sequences in plant transformation. Bacterial strains including strains with T-DNA integrated into the chromosomal DNA reduce the copy number and vector backbone sequences. Chromosomal integration vectors to integrate T-DNA into a specific locus of chromosome are disclosed.
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