Bruce D Gaylinn

US Patent:

7078514, Jul 18, 2006

Filed:

Jun 12, 2000

Appl. No.:

10/009643

Inventors:

Michael O. Thorner - North Garden VA, US
Bruce David Gaylinn - Louisa VA, US
Andrew Alan Toogood - Solihull, GB
Steve Harvey - Edmonton, Alberta, CA

International Classification:

C07K 14/72

US Classification:

536 235, 530350, 530399

Abstract:

The present invention relates to chicken growth hormone releasing hormone (GHRH), its corresponding receptor, and nucleic acid sequences encoding these proteins. More particularly the present invention is directed to the use of the chicken GHRH hormone and its corresponding GHRH receptor to enhance the production of larger, leaner chickens and other avian species used for meat production.

US Patent:

55916414, Jan 7, 1997

Filed:

Sep 15, 1992

Appl. No.:

7/946232

Inventors:

Michael O. Thorner - North Garden VA
Bruce D. Gaylinn - Trevilians VA
John R. Zysk - French Town NJ
Kevin R. Lynch - Charlottesville VA
Jeffrey K. Harrison - Keswick VA

Assignee:

American Cyanamid Company - Wayne NJ
The University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12N 1512
C07K 14705

US Classification:

435 691

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.

US Patent:

59731141, Oct 26, 1999

Filed:

Jun 23, 1992

Appl. No.:

7/902826

Inventors:

Michael Oliver Thorner - North Garden VA
Bruce David Gaylinn - Trevilians VA
John Ronald Zysk - French Town NJ
Cecil Mark Eppler - Langhorne PA

Assignee:

The University of Virginia Patent Foundation - Charlottesville VA
American Cyanamid Company - Wayne NJ

International Classification:

C07K 14705

US Classification:

530350

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor.

US Patent:

58521879, Dec 22, 1998

Filed:

Jun 12, 1996

Appl. No.:

8/660963

Inventors:

Michael O. Thorner - North Garden VA
Bruce D. Gaylinn - Trevilians VA
Reiko Horikawa - Tokyo, JP
Charles E. Lyons - Charlottesville VA

Assignee:

The University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12N 1512
C12N 1510
C07K 1472

US Classification:

536 235

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromatography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line. In addition, molecular cloning of the ovine GHRH-R is provided.

US Patent:

55830102, Dec 10, 1996

Filed:

Jun 10, 1993

Appl. No.:

8/073799

Inventors:

William R. Baumbach - Hopewell NJ
John R. Zysk - Frenchtown NJ
Michael O. Thorner - North Garden VA
Bruce D. Gaylinn - Trevilians VA
Kevin R. Lynch - Charlottesville VA
Jeffrey K. Harrison - Gainesville FL

Assignee:

American Cyanamid Company - Wayne NJ
The University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12N 1512
C07K 14705

US Classification:

435 691

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.

US Patent:

56440464, Jul 1, 1997

Filed:

May 1, 1995

Appl. No.:

8/432043

Inventors:

Michael Oliver Thorner - North Garden VA
Bruce David Gaylinn - Trevilians VA
Jeffrey K. Harrison - Gainesville FL
Kevin R. Lynch - Charlottesville VA
John Ronald Zysk - Frenchtown NJ

Assignee:

The University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12N 1512

US Classification:

536 235

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromatography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.

US Patent:

56124708, Mar 18, 1997

Filed:

Sep 18, 1992

Appl. No.:

7/947672

Inventors:

Michael O. Thorner - North Garden VA
Bruce D. Gaylinn - Trevilians VA
John R. Zysk - French Town NJ
Kevin R. Lynch - Charlottesville VA
Jeffrey K. Harrison - Keswick VA

Assignee:

American Cyanamid Company - Wayne NJ
The University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12N 1512

US Classification:

536 235

Abstract:

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.

US Patent:

20210373011, Dec 2, 2021

Filed:

Aug 7, 2017

Appl. No.:

16/322679

Inventors:

- Charlottesville VA, US
Alexander L. KLIBANOV - Charlottesville VA, US
Bruce D. GAYLINN - Louisa VA, US

International Classification:

G01N 33/543
G01N 33/68

Abstract:

Lanthanide chelate lipid nanoparticles, and methods of their synthesis and use are described. Biological molecules labeled with the lipid nanoparticles, useful in bioaffinity assays with improved sensitivities are also described.