Camille P Dubois

US Patent:

20120142105, Jun 7, 2012

Filed:

Oct 3, 2011

Appl. No.:

13/251960

Inventors:

Sung-Yong Harrison Yoon - Lake Oswego OR, US
Young Chul Park - Beaverton OR, US
Amy McDonald - Oregon City OR, US
Camille Pierre Dubois - Milwaukie OR, US
Sonia Lall - Portland OR, US

Assignee:

DianaPlantSciences, Inc. - Portland OR

International Classification:

C12N 5/00

US Classification:

435410

Abstract:

A method of preparing cocoa oligomeric procyanidins from cocoa cell cultures grown in the presence of monosaccharide can increase production of procyanidins as follows: culturing cells sufficient to result in production of cocoa oligomeric procyanidins at a first rate; and introducing a monosaccharide to the cells sufficient for inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that his higher than the first rate. The method can further include extracting the cocoa oligomeric procyanidins from the cells, and such extracting can occur between 1 day to 21 days after introduction of the monosaccharide. Optionally, the monosaccharide can be introduced in an amount about 0.5% to about to about 20% by volume of the culture medium. The monosaccharide can be glucose, sucrose, fructose, or the like. The monosaccharide can be introduced during or after a last phase of an exponential growth state.