Casper W Vroemen

US Patent:

20120252101, Oct 4, 2012

Filed:

Mar 2, 2012

Appl. No.:

13/410486

Inventors:

Claudine Y. Chang - Palo Alto CA, US
Clement Choy - Palo Alto CA, US
Melodie Estabrook - Palo Alto CA, US
Mansi Goyal - Palo Alto CA, US
Thomas P. Graycar - Palo Alto CA, US
Victoria E. Huang - Palo Alto CA, US
Brian E. Jones - Palo Alto CA, US
Marc Kolkman - Palo Alto CA, US
Karsten M. Kragh - Palo Alto CA, US
Chris Leeflang - Palo Alto CA, US
Scott D. Power - Palo Alto CA, US
Sandra W. Ramer - Palo Alto CA, US
Andrew SHAW - Palo Alto CA, US
Casper Vroemen - Palo Alto CA, US
Walter Weyler - Palo Alto CA, US

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12N 1/21
C12N 1/15
C12N 15/56
C12N 15/63

US Classification:

43525231, 4353201, 43525411, 43525235, 43525233, 43525234, 536 232

Abstract:

Variants of sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

US Patent:

20200354705, Nov 12, 2020

Filed:

Mar 9, 2020

Appl. No.:

16/812641

Inventors:

- PALO ALTO CA, US
VIKTOR YURYEVICH ALEKSEYEV - Palo Alto CA, US
CASPER WILLEM VROEMEN - Palo Alto CA, US
DAVID A. ESTELL - Palo Alto CA, US

International Classification:

C12N 9/34
C12P 19/14

Abstract:

Aspects of the present disclosure include glucoamylase (GA) variants having at least one improved property over a parent GA, compositions containing the GA variants, nucleic acids encoding the GA variants, and methods for producing and using the same. In some aspects, the GA variant is a variant of a parent GA from

US Patent:

20170306309, Oct 26, 2017

Filed:

Apr 12, 2017

Appl. No.:

15/485509

Inventors:

- Copenhagen, DK
Peter Edvard DEGN - Egå, DK
Igor NIKOLAEV - Noordwijk, NL
Viktor ALEKSEYEV - Palo Alto CA, US
Casper Willem VROEMEN - Palo Alto CA, US
Daniel TORRES PAZMINO - Overrijn 12, NL
Neville Marshall FISH - Stockport, GB

Assignee:

DuPont Nutrition Biosciences APS - Copenhagen

International Classification:

C12N 9/34
C12C 11/00
C12C 7/04

Abstract:

The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable for use for example in the production of a food, beverage (e.g. beer), feed, biochemical, or biofuel. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.

US Patent:

20160272958, Sep 22, 2016

Filed:

Dec 3, 2014

Appl. No.:

15/037040

Inventors:

- Palo Alto CA, US
Viktor Yuryevich Alekseyev - Palo Alto CA, US
Casper Willem Vroemen - Palo Alto CA, US
David A. Estell - San Francisco CA, US

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12N 9/34
C12P 19/20

Abstract:

Aspects of the present disclosure include glucoamylase (GA) variants having at least one improved property over a parent GA, compositions containing the GA variants, nucleic acids encoding the GA variants, and methods for producing and using the same. In some aspects, the GA variant is a variant of a parent GA from

US Patent:

20160040202, Feb 11, 2016

Filed:

Dec 5, 2013

Appl. No.:

14/649170

Inventors:

- Palo Alto CA, US
Martijn Scheffers - Leiden, NL
Marco Van Brussel - Zoetmeer, NL
Casper Vroemen - Palo Alto CA, US
Bo Zhang - Shanghai, CN
Zhenghong Zhang - Shanghai, CN

International Classification:

C12P 19/02
A23L 2/38
C12C 5/00
C12P 19/14
C12P 7/14

Abstract:

A fungal alpha-amylase is provided from (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

US Patent:

20160032338, Feb 4, 2016

Filed:

Dec 5, 2013

Appl. No.:

14/649173

Inventors:

- Palo Alto CA, US
Martijn Scheffers - Leiden, NL
Marco Van Brussel - Zoetermeer, NL
Casper Vroemen - Palo Alto CA, US
Bo Zhang - Shanghai, CN
Zhengong Zhang - Shanghai, CN

International Classification:

C12P 19/14
C12P 19/02
C12C 11/00
C12N 9/44

Abstract:

A fungal alpha-amylase is provided from (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

US Patent:

20160010128, Jan 14, 2016

Filed:

Dec 6, 2013

Appl. No.:

14/649807

Inventors:

- Palo Alto CA, US
Ling Hua - Shanghai, CN
Martijn Scheffers - Leiden, NL
Marco Van Brussel - Zoetermeer, NL
Casper Vroemen - Palo Alto CA, US
Bo Zhang - Shanghai, CN

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12P 19/14
C12P 19/02
C12P 7/14

Abstract:

A fungal alpha-amylase is provided from (AtAmyl). AtAmyl has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

US Patent:

20150376668, Dec 31, 2015

Filed:

Dec 6, 2013

Appl. No.:

14/649805

Inventors:

- Palo Alto CA, US
Ling Hua - Hockessin DE, US
Martijn Scheffers - Leiden, NL
Marco Van Brussel - Zoetermeer, NL
Casper Vroemen - Palo Alto CA, US
Bo Zhang - Shanghai, CN

International Classification:

C12P 19/14
C12P 7/14
C12P 19/02

Abstract:

A fungal alpha-amylase is provided from (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from . This facilitates the utilization of the ligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.