Skills:
SKILLS AND TECHNIQUES -Molecular Biology, Genetics -Execution, trouble-shooting, and analysis of the following molecular biological techniques: -Sanger sequencing (STR and sequencing analysis); TaqMan genotyping, TaqMan gene expression; Western blot; cell culture and sterile technique; DNA extraction from blood, saliva, and tissue; RNA extraction from blood, saliva, and tissue; PCR amplification; reverse transcription; agarose and polyacrylamide gel electrophoresis; real time quantitative PCR (RT-qPCR);, restriction digest; cloning; transfection; bisulfite conversion of DNA; protein expression in, and purification from, E. coli; protein preparation from tissue; Western blot; immunofluorescence; immunohistochemistry; cell culture and sterile technique, microscopy, including experience with fluorescent, inverted, and confocal microscopes -Writing skills -8 years experience preparing and revising scientific manuscripts, grants, and technical protocols -Leadership, innovation, and communication skills -Experience training and supervising laboratory personnel of all professional and educational levels including undergraduates, medical students, graduate students, medical fellows and other post-docs -Project management skills including defining a project, producing a budget, executing a plan, reporting on progress, and evaluating the outcome -Excellent problem-solving skills and expertise in defining a question, designing and carrying out experiments, trouble-shooting obstacles, and analyzing and interpreting results -Excellent interpersonal and communication skills, both written and verbal