Cecilia U Smith

US Patent:

6387622, May 14, 2002

Filed:

Oct 27, 1999

Appl. No.:

09/403919

Inventors:

Olavi Siiman - Davie FL
Alexander Burshteyn - Hialeah FL
Ravindra Mylvaganam - Hollywood FL
Robert Raynor - Cooper City FL
Patricia Roth - Pembroke Pines FL
Cecilia Smith - Miami FL
Julie Wilkinson - Weston FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3368

US Classification:

435 6, 435 722, 435 723, 435 724, 435 725, 435 72, 436172, 436512, 436513, 436529, 436800, 436805, 5303911, 5303913, 5303915

Abstract:

Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.

US Patent:

7354773, Apr 8, 2008

Filed:

May 14, 2003

Appl. No.:

10/437695

Inventors:

Oilda Rubio - Miami FL, US
Carlos Aparicio - Miami FL, US
John A. Maples - Miami FL, US
Julie Wilkinson - Fort Lauderdale FL, US
Cecilia Smith - Miami FL, US
Frank J. Lucas - Boca Raton FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 1/18

US Classification:

436177, 435 2, 435 725, 435 405, 435366, 435372, 435398, 435400, 435401, 4352872, 435971, 436521, 436522, 436536, 436538, 436546, 436 17, 436 45, 436 47

Abstract:

A method and apparatus for preparing biological cell samples for intracellular analysis. The invention is based upon the recognition that many of the steps of the conventional methods for such sample preparation can be eliminated, leading to a process that readily lends itself to automation and the advantages associated therewith. The method of the invention comprises the steps of (a) cell-fixation, (b) permeabilization and (c) staining (or labeling) of intracellular molecules of interest by probes that are readily detectable by flow cytometric techniques, all without any intervening cell-washing (and re-suspension) steps. Rather, the single cell-washing step is effected after these three steps have been carried out. Preferably, the washing step is carried out by passing the fixed, permeabilized and stained cell sample through a semi-permeable membrane that serves to filter out (by transmission) interferants to waste while retaining the cells of interest.

US Patent:

20070015134, Jan 18, 2007

Filed:

Jul 15, 2005

Appl. No.:

11/183132

Inventors:

John Maples - Miami FL, US
Rhonda Mills - Pembroke Pines FL, US
Daniel Flagler - Miami FL, US
Cecilia Smith - Lake Placid FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 1/00
G01N 33/567
G01N 33/53

US Classification:

435004000, 435007200, 977900000

Abstract:

The invention relates to quantitative stabilized cell reference control products, their methods of manufacture, and their methods of use. Receptors, antigens, and ligands associated with stabilized cells may be quantitated by the methods of the present invention. Those quantitated stabilized cells may then be used to determine the receptor, antigen, and/or ligand density of unknown samples.

US Patent:

58917411, Apr 6, 1999

Filed:

May 16, 1997

Appl. No.:

8/857941

Inventors:

Olavi Siiman - Davie FL
Cecilia Smith - Miami FL
Patricia Roth - Pembroke Pines FL
Alexander Burshteyn - Hialeah FL
Robert Raynor - Cooper City FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 33548

US Classification:

436529

Abstract:

The present application discloses dextran crosslinked, antibody-phycobiliprotein conjugates containing up to twenty phycobiliprotein per dextran molecule which conjugates are able to achieve amplification of fluorescence intensity over direct antibody-phycobiliprotein labeled cells of two fold or greater.