Cesario F Bianchi

US Patent:

55996728, Feb 4, 1997

Filed:

Dec 8, 1994

Appl. No.:

8/351748

Inventors:

Peng Liang - Watertown MA
Arthur B. Pardee - Brookline MA
Cesario F. Bianchi - Boston MA

Assignee:

Dana-Farber Cancer Institute, Inc. - Boston MA
The President and Fellows of Harvard College - Cambridge MA

International Classification:

C07H 2104
C12P 1934
C12Q 168

US Classification:

435 6

Abstract:

A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence that is substantially identical to the sequence of a mRNA having a known sequence.