Charles M Richardson

US Patent:

20020172972, Nov 21, 2002

Filed:

May 15, 2002

Appl. No.:

10/146872

Inventors:

Stanley Tabor - Brookline MA, US
Charles Richardson - Newton MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Methods and kits for rendering inert contaminating nucleic acids are provided. The methods and kits include an enzyme capable of digesting the contaminating nucleic acids, an activator which activates the enzyme, and an inactivating agent capable of binding to or displacing the activator from the enzyme thus rendering the enzyme inactive.

US Patent:

20050164213, Jul 28, 2005

Filed:

Nov 7, 2003

Appl. No.:

10/813693

Inventors:

Stanley Tabor - Brookline MA, US
Charles Richardson - Newton MA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

US Patent:

54985234, Mar 12, 1996

Filed:

Jul 14, 1994

Appl. No.:

8/275339

Inventors:

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 168
C12P 1934
G01N 3350
C12N 914

US Classification:

435 6

Abstract:

A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

US Patent:

56747160, Oct 7, 1997

Filed:

Apr 13, 1995

Appl. No.:

8/422147

Inventors:

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 1934
C12Q 168
C12Q 170
C07K 314

US Classification:

435 911

Abstract:

A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

US Patent:

D2934728, Dec 29, 1987

Filed:

Jul 3, 1985

Appl. No.:

6/752217

Inventors:

Anthony Chrones - Warwick RI
Charles C. Richardson - North Kingstown RI

Assignee:

Gerber Products Company - Fremont MI

US Classification:

D24 36

US Patent:

59726032, Oct 26, 1999

Filed:

May 31, 1996

Appl. No.:

8/656555

Inventors:

Ella Bedford - Brookline MA
Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 168
C12Q 170
C07K 100

US Classification:

435 6

Abstract:

Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

US Patent:

49421301, Jul 17, 1990

Filed:

Dec 14, 1987

Appl. No.:

7/132569

Inventors:

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

Assignee:

President & Fellows of Harvard College - Cambridge MA

International Classification:

C12N 912
C12N 1500
C07H 1512

US Classification:

435194

US Patent:

52664660, Nov 30, 1993

Filed:

Sep 4, 1992

Appl. No.:

7/940531

Inventors:

Stanley Tabor - Cambridge MA
Charles C. Richardson - Chestnut Hill MA

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 1934
C12N 912
C12Q 168

US Classification:

435 915

Abstract:

This invention relates to T7-type DNA polymerases and methods for using them.