Charles H Rundle

US Patent:

20070212332, Sep 13, 2007

Filed:

Aug 10, 2006

Appl. No.:

11/503365

Inventors:

David Baylink - Redlands CA, US
Charles Rundle - Redlands CA, US
Donna Strong - Beaumont CA, US
Shin-Tai Chen - Colton CA, US
Thomas Linkhart - Redlands CA, US

International Classification:

A61K 48/00

US Classification:

424093200, 514044000

Abstract:

Vectors, such as retroviral vectors and transposon-based nonviral vectors, are disclosed herein that can be used to target transgene expression to the proliferating periosteal cells and cells in the marrow space after bone fracture. In one embodiment, these vectors include a human Cox-2 gene that is modified to improve mRNA stability and protein translation by truncating the 3′ untranslated region (UTR). In addition, in some embodiments, the native translation signal is replaced with an optimized Kozak sequence. These vectors can be used alone or with vectors expressing BMP2/4, FGF-2, or LMP-1 gene to repair bone fractures and increase prostaglandin secretion. Methods for identifying agents that accelerate bone repair are also disclosed.

US Patent:

20150240219, Aug 27, 2015

Filed:

Apr 15, 2015

Appl. No.:

14/687787

Inventors:

- Baltimore MD, US
- Loma Linda CA, US
Charles Rundle - Redlands CA, US
Donna Strong - Beaumont CA, US
Shin-Tai Chen - Colton CA, US
Thomas Linkhart - Redlands CA, US

International Classification:

C12N 9/02

Abstract:

Vectors, such as retroviral vectors and transposon-based nonviral vectors, are disclosed herein that can be used to target transgene expression to the proliferating periosteal cells and cells in the marrow space after bone fracture. In one embodiment, these vectors include a human Cox-2 gene that is modified to improve mRNA stability and protein translation by truncating the 3′ untranslated region (UTR). In addition, in some embodiments, the native translation signal is replaced with an optimized Kozak sequence. These vectors can be used alone or with vectors expressing BMP2/4, FGF-2, or LMP-1 gene to repair bone fractures and increase prostaglandin secretion. Methods for identifying agents that accelerate bone repair are also disclosed.